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Human serum amyloid A2 (SAA2) ELISA kit

  • 中文名: 人血清淀粉样蛋白A2(SAA2)酶联免疫试剂盒
货号: IPD-EL020657HU
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人血清淀粉样蛋白A2(SAA2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的SAA2含量。SAA2即血清淀粉样蛋白A2,属于急性时相蛋白。在炎症、感染等刺激下其表达水平会急剧上升。研究机制方面,它参与免疫调节,可趋化免疫细胞。还与代谢紊乱、心血管疾病等发病机制有一定联系,是潜在的疾病诊断与治疗靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL,为探索炎症相关疾病机制(如动脉粥样硬化、类风湿性关节炎等)提供可靠工具,亦可用于药物干预或基因编辑模型中SAA2表达变化的科研评估,满足体外实验、动物模型及细胞研究等多场景需求。适用于血清、血浆等体液样本中SAA2的动态监测,或组织匀浆中蛋白表达的定量分析。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    N/A
  • 缩写:
    SAA2
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.312 ng/mL-20 ng/mL
  • 灵敏度:
    0.078 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human SAA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:50

    Average %

    93


    Range %

    88-99


    1:100

    Average %

    89


    Range %

    82-96


    1:200

    Average %

    99


    Range %

    94-105


    1:400

    Average %

    92


    Range %

    86-98


  • 回收率:

    The recovery of human SAA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    98

    91-104


    EDTA plasma (n=4)

    91

    84-97


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    20

    2.175

    2.234

    2.205

    2.066


    10

    1.784

    1.667

    1.726

    1.587


    5

    1.303

    1.308

    1.306

    1.167


    2.5

    0.829

    0.869

    0.849

    0.710


    1.25

    0.577

    0.601

    0.589

    0.450


    0.625

    0.432

    0.453

    0.443

    0.304


    0.312

    0.267

    0.280

    0.274

    0.135


    0

    0.143

    0.135

    0.139




  • 货期:
    3-5 working days

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