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产品描述：人可溶性血小板内皮细胞粘附分子1(sPECAM-1/sCD31)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的sPECAM1/sCD31含量。sPECAM1/sCD31即可溶性血小板内皮细胞黏附分子-1/可溶性分化簇31。它在血管生成、炎症反应等生理病理过程中发挥关键作用。研究机制主要聚焦于其在细胞黏附、迁移中的功能，以及与心血管疾病、肿瘤等疾病发生发展的关联。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品适用于体外研究血管生物学机制、炎症微环境调控以及药物干预实验中 sPECAM - 1 的动态变化监测，为心血管疾病模型构建、免疫调节机制探索及生物标志物筛选等科研领域提供标准化检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：PECAM1; Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31
缩写：sPECAM1/sCD31
Uniprot No.：P16284
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SPECAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:200 | Average % | 88 | ||||
| Range % | 84-93 | |||||
| 1:400 | Average % | 100 | ||||
| Range % | 95-110 | |||||
| 1:800 | Average % | 90 | ||||
| Range % | 85-97 | |||||
回收率：
| The recovery of human SPECAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 80-90 | ||||
| EDTA plasma (n=4) | 93 | 89-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.4682.3242.3962.302 10001.9311.8521.8921.798 5001.2241.1761.2001.106 2500.5680.6020.5850.491 1250.3330.3410.3370.243 62.50.2370.2300.2340.140 31.250.1660.1620.1640.070 00.0930.0940.094 | ||||||
货期：3-5 working days
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