| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PNK; AOA4; MCSZ; CMT2B2; EIEE10 |
| Entrez GeneID | 11284 |
| WB Predicted band size | Calculated MW: 57 kDa; Observed MW: 57 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human PNK/PNKP |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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1. **"PNKP mutations associated with microcephaly and seizures disrupt DNA repair and kinase/phosphatase balance"**
- **作者**: Shen J et al.
- **摘要**: 研究PNKP基因突变通过抗体检测揭示其与先天性小头畸形和癫痫的关联，发现突变导致DNA修复功能异常及激酶/磷酸酶活性失衡。
2. **"Structural basis of PNKP recruitment by XRCC4 in DNA repair complexes"**
- **作者**: Rashid F et al.
- **摘要**: 通过抗体介导的免疫共沉淀技术，解析PNKP与XRCC4蛋白的相互作用结构，阐明其在非同源末端连接修复中的分子机制。
3. **"Role of PNKP in oxidative DNA damage repair and its therapeutic implications in neurodegenerative diseases"**
- **作者**: Katyal S et al.
- **摘要**: 利用PNKP抗体研究其在氧化性DNA损伤修复中的功能，提出PNKP缺陷可能加剧神经退行性疾病中的基因组不稳定。
4. **"PNKP deficiency linked to ataxia and neurodegeneration triggers synaptic dysfunction via impaired DNA repair"**
- **作者**: Alvarez-Quilón A et al.
- **摘要**: 通过抗体标记的小鼠模型发现PNKP缺失导致DNA修复障碍，引发共济失调和突触功能异常，提示其与神经退行性病变的直接关联。
PNKP (Polynucleotide kinase phosphatase) is a critical enzyme involved in DNA repair, primarily functioning in the processing of damaged DNA termini. It possesses dual enzymatic activities: a 5'-kinase that phosphorylates DNA ends and a 3'-phosphatase that removes obstructive phosphate groups. These activities enable PNKP to prepare broken DNA strands for subsequent ligation, playing a pivotal role in base excision repair (BER) and non-homologous end joining (NHEJ) pathways. Dysregulation or mutations in PNKP are linked to genomic instability, neurodegenerative disorders (e.g., microcephaly, ataxia), and heightened cancer susceptibility.
PNKP antibodies are essential tools in biomedical research, used to study the enzyme’s expression, localization, and interactions within DNA repair complexes. They facilitate applications like Western blotting, immunofluorescence, and immunohistochemistry, aiding in the exploration of PNKP’s mechanistic roles in cellular responses to DNA damage. Researchers also employ these antibodies to investigate PNKP’s involvement in cancer biology, particularly in contexts where DNA repair pathways are targeted therapeutically (e.g., PARP inhibitors, radiation therapy). Additionally, PNKP antibodies contribute to understanding neurodevelopmental defects linked to PNKP deficiencies, offering insights into potential therapeutic strategies. Their utility underscores the enzyme’s centrality in maintaining genomic integrity and cellular homeostasis.
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