| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RSPO1 |
| Uniprot No | Q2MKA7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-263aa |
| 氨基酸序列 | SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RSPO1重组蛋白的模拟参考文献示例(内容基于公开研究领域知识整合，非真实文献)：
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1. **"R-spondin1/LGR5 signaling regulates intestinal stem cell maintenance"**
*作者：Tao, S., et al.*
**摘要**：研究利用重组RSPO1蛋白激活Wnt/β-catenin信号通路，证实其可显著促进肠道类器官的增殖与分化，揭示了RSPO1在维持肠道干细胞稳态中的关键作用。
2. **"Recombinant RSPO1 enhances antitumor immunity by modulating tumor microenvironment"**
*作者：Kim, J.H., et al.*
**摘要**：通过动物模型证明，重组RSPO1蛋白通过增强Wnt信号通路和调节免疫细胞浸润，抑制肿瘤生长，为癌症免疫治疗提供了新策略。
3. **"Structural basis of RSPO1 recognition by LGR5 receptors"**
*作者：Wang, Y., et al.*
**摘要**：通过X射线晶体学解析重组RSPO1蛋白与受体LGR5的复合物结构，阐明了二者结合的关键氨基酸位点，为靶向药物设计提供结构基础。
4. **"RSPO1 recombinant protein promotes hair follicle regeneration"**
*作者：Chen, L., et al.*
**摘要**：体外实验显示，重组RSPO1蛋白通过激活毛囊干细胞Wnt通路，显著加速皮肤损伤模型中毛囊再生，提示其在再生医学中的应用潜力。
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注：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索关键词(如"RSPO1 recombinant protein")获取。
R-spondin 1 (RSPO1) is a secreted glycoprotein belonging to the R-spondin protein family, which comprises four members (RSPO1-4) known for their critical roles in modulating Wnt/β-catenin signaling. This pathway is essential for embryonic development, stem cell maintenance, and tissue regeneration. RSPO1 enhances Wnt signaling by binding to leucine-rich repeat-containing G-protein-coupled receptors (LGR4/5/6) and neutralizing transmembrane E3 ubiquitin ligases ZNRF3/RNF43. which degrade Wnt receptors. This interaction stabilizes Wnt receptors on the cell surface, amplifying Wnt signal transduction and promoting cellular proliferation, differentiation, and tissue homeostasis.
RSPO1 is particularly significant in gastrointestinal stem cell regulation, skin development, and female sexual differentiation. Mutations in the RSPO1 gene are linked to 46.XX testicular/ovotesticular disorders of sex development, highlighting its role in ovarian development. Recombinant RSPO1 proteins are engineered using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications. They are purified via affinity chromatography and validated for bioactivity in cell-based assays, such as Wnt signaling activation or organoid growth support.
In research, recombinant RSPO1 is widely used to expand stem cells in organoid cultures, model diseases, and study regenerative mechanisms. Its therapeutic potential is being explored in conditions involving impaired Wnt signaling, including intestinal injuries, hair loss, and certain cancers. However, aberrant RSPO1 expression is also associated with tumorigenesis, underscoring the need for precise regulation. Overall, RSPO1 serves as a vital tool in both basic research and translational applications targeting tissue repair and disease modulation.
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