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产品描述：兔肌酸激酶同工酶MB(CK-MB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CK-MB含量。CK-MB即肌酸激酶同工酶，主要存在于心肌中。当心肌损伤时，CK - MB会释放入血，其水平变化可反映心肌损伤程度。研究多围绕其作为心肌损伤早期敏感标志物展开，监测它对急性心肌梗死等疾病的诊断、治疗及预后评估有重要意义。试剂盒检测范围为1.56 mU/mL-100 mU/mL，适用于心血管疾病机制研究、药物心脏毒性评估及动物模型中心肌损伤的动态监测；适用于心肌缺血再灌注损伤、药物干预效果评价、病理模型建立等科研场景，助力心血管相关疾病的基础研究与转化探索本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：CK-MB
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 mU/mL-100 mU/mL
灵敏度：0.39 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-100 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 96-107 | |||||
回收率：
| The recovery of rabbit CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 83-96 | ||||
| EDTA plasma (n=4) | 92 | 88-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2AverageCorrected1002.8892.9842.9372.833502.4352.5662.5012.397251.8151.9231.8691.76512.51.0121.0271.0200.9166.250.5120.5060.5090.4053.120.3240.3110.3180.2141.560.1810.1780.1800.07600.1040.1030.104 | ||||||
货期：3-5 working days
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