| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LILRB2 |
| Uniprot No | Q8N423 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-458aa |
| 氨基酸序列 | QTGTIPKPTLWAEPDSVITQGSPVTLSCQGSLEAQEYRLYREKKSASWIT RIRPELVKNGQFHIPSITWEHTGRYGCQYYSRARWSELSDPLVLVMTGAY PKPTLSAQPSPVVTSGGRVTLQCESQVAFGGFILCKEGEDEHPQCLNSQP HARGSSRAIFSVGPVSPNRRWSHRCYGYDLNSPYVWSSPSDLLELLVPGV SKKPSLSVQPGPVVAPGESLTLQCVSDVGYDRFVLYKEGERDLRQLPGRQ PQAGLSQANFTLGPVSRSYGGQYRCYGAYNLSSEWSAPSDPLDILITGQI HGTPFISVQPGPTVASGENVTLLCQSWRQFHTFLLTKAGAADAPLRLRSI HEYPKYQAEFPMSPVTSAHAGTYRCYGSLNSDPYLLSHPSEPLELVVSGP SMGSSPPPTGPISTPAGPEDQPLTPTGSDPQSGLGRHVDHHHHHH |
| 预测分子量 | 49 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LILRB2重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*Structural basis for LILRB2 recognition of HLA-G in immune tolerance*
**作者**：Liu J, et al.
**摘要**：该研究解析了LILRB2重组蛋白与配体HLA-G的复合物晶体结构，揭示了LILRB2通过特定免疫球蛋白样结构域与HLA-G结合的分子机制，为靶向LILRB2/HLA-G互作在肿瘤免疫逃逸中的治疗策略提供依据。
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2. **文献名称**：*LILRB2 suppresses T cell activation via SHP-1 recruitment in cancer immunotherapy*
**作者**：Chen X, et al.
**摘要**：通过体外表达LILRB2重组蛋白，研究发现其与肿瘤微环境中的配体结合后，招募磷酸酶SHP-1抑制T细胞受体信号通路，提出阻断LILRB2可增强抗肿瘤免疫应答。
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3. **文献名称**：*Recombinant LILRB2 extracellular domain modulates myeloid-derived suppressor cell function in sepsis*
**作者**：Wang Y, et al.
**摘要**：利用重组LILRB2胞外段蛋白，实验证明其可通过竞争性抑制内源性LILRB2.逆转脓毒症模型中髓系抑制细胞(MDSCs)的免疫抑制功能，改善过度炎症反应。
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4. **文献名称**：*LILRB2 promotes AML progression via APOE-mediated signaling*
**作者**：Deng M, et al.
**摘要**：研究通过重组LILRB2蛋白验证其与载脂蛋白E(APOE)的相互作用，阐明LILRB2在急性髓系白血病(AML)中通过激活PI3K/AKT通路促进肿瘤细胞增殖的机制。
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以上文献均聚焦LILRB2重组蛋白在结构解析、免疫调控及疾病治疗中的功能研究。
LILRB2 (leukocyte immunoglobulin-like receptor B2), also known as CD85d or ILT4. is a transmembrane glycoprotein belonging to the leukocyte immunoglobulin-like receptor (LILR) family. It is primarily expressed on myeloid-derived immune cells, including monocytes, macrophages, dendritic cells, and neutrophils, as well as natural killer (NK) cells. Structurally, LILRB2 contains extracellular immunoglobulin-like domains that mediate ligand binding, a transmembrane region, and cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) responsible for transmitting inhibitory signals.
Functionally, LILRB2 acts as an immune checkpoint receptor by binding to MHC class I molecules (HLA-A, -B, -C) and other ligands like angiopoietin-like proteins (ANGPTLs). This interaction triggers ITIM phosphorylation, recruiting phosphatases such as SHP-1/SHP-2 to dampen immune cell activation. In physiological conditions, LILRB2 helps maintain immune tolerance and prevent excessive inflammation. However, in pathological contexts like cancer, tumors exploit LILRB2-mediated signaling to evade immune surveillance. Overexpression of LILRB2 ligands in the tumor microenvironment suppresses anti-tumor responses by inhibiting antigen presentation and T-cell activation.
Recombinant LILRB2 proteins are engineered for research and therapeutic development. These proteins typically include the extracellular domain produced in mammalian or insect cell systems to ensure proper glycosylation and ligand-binding capacity. They serve as critical tools for studying receptor-ligand interactions, screening inhibitory antibodies, and designing targeted therapies. Recent studies explore blocking LILRB2 to reverse immune suppression in cancers or modulate inflammatory diseases. Additionally, LILRB2 is implicated in infectious diseases, including viral immune evasion, making it a multifunctional target in immunotherapy. Its dual role in homeostasis and disease underscores both its biological complexity and therapeutic potential.
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