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产品描述:人HLAⅡ类组织相容性抗原,DRα链(HLA-DRA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的HLA-DRA含量。HLA - DRA是一种重要靶点。它属于人类白细胞抗原基因家族,在免疫系统中发挥关键作用,参与抗原呈递过程。目前研究围绕其在自身免疫疾病、肿瘤免疫等方面的机制,旨在明确其作用途径,为相关疾病的诊断、治疗及药物研发提供依据。试剂盒检测范围为18.75 pg/mL-1200 pg/mL,适用于基础免疫学研究、疾病发生机制探索以及生物治疗药物(如免疫调节剂或单克隆抗体)的体外效果评估,为科研人员提供稳定可靠的定量分析工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:DASS-397D15.1 ELISA Kit; DR alpha chain ELISA Kit; DR alpha chain precursor ELISA Kit; DRA_HUMAN ELISA Kit; DRB1 ELISA Kit; DRB4 ELISA Kit; FLJ51114 ELISA Kit; Histocompatibility antigen HLA DR alpha ELISA Kit; Histocompatibility antigen HLA-DR alpha ELISA Kit; HLA class II histocompatibility antigen ELISA Kit; HLA class II histocompatibility antigen DR alpha chain ELISA Kit; HLA DR1B ELISA Kit; HLA DR3B ELISA Kit; HLA DRA ELISA Kit; HLA DRA1 ELISA Kit; HLA DRB1 ELISA Kit; HLA DRB3 ELISA Kit; HLA DRB4 ELISA Kit; HLA DRB5 ELISA Kit; HLA-DR histocompatibility type ELISA Kit; HLA-DRA ELISA Kit; HLADR4B ELISA Kit; HLADRA1 ELISA Kit; HLADRB ELISA Kit; Major histocompatibility complex class II DR alpha ELISA Kit; Major histocompatibility complex class II DR beta 1 ELISA Kit; Major histocompatibility complex class II DR beta 3 ELISA Kit; Major histocompatibility complex class II DR beta 4 ELISA Kit; Major histocompatibility complex class II DR beta 5 ELISA Kit; MGC117330 ELISA Kit; MHC cell surface glycoprotein ELISA Kit; MHC class II antigen DRA ELISA Kit; MHC II ELISA Kit; MLRW ELISA Kit; OTTHUMP00000029406 ELISA Kit; OTTHUMP00000029407 ELISA Kit
缩写:HLA-DRA
Uniprot No.:P01903
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:18.75 pg/mL-1200 pg/mL
灵敏度:4.68 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Immunology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human HLA-DRA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 91 | ||||
Range % | 84-98 | |||||
1:2 | Average % | 91 | ||||
Range % | 85-95 | |||||
1:4 | Average % | 96 | ||||
Range % | 92-102 | |||||
1:8 | Average % | 103 | ||||
Range % | 96-108 |
回收率:
The recovery of human HLA-DRA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 92 | 88-96 | ||||
EDTA plasma (n=4) | 96 | 90-101 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
pg/mlOD1OD2AverageCorrected12002.2472.2352.2412.1216001.4921.5891.5411.4213000.9740.9990.9870.8671500.6220.6320.6270.507750.4320.4120.4220.30237.50.2890.3100.3000.18018.750.2080.1980.2030.08300.1210.1190.120 |
货期:3-5 working days
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