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产品描述：仓鼠白介素10(IL10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL10含量。IL10是一种细胞因子，其背景在于它具有免疫调节作用，能抑制炎症反应。研究机制上，它可作用于多种免疫细胞，通过与特异性受体结合，激活下游信号通路，调控细胞因子的产生与释放，在免疫性疾病、感染等研究领域备受关注。试剂盒检测范围为6.25 pg/mL-400 pg/mL，IL10水平变化常被用于评估感染性疾病、自身免疫病等病理模型的免疫状态；尤其适用于仓鼠炎症模型研究、疫苗免疫效果评价或药物干预实验中的生物标志物分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CSIF, IL-10, IL10A, MGC126450, MGC126451, TGIF, cytokine synthesis inhibitory factor
缩写：IL10
种属：Mesocricetus auratus (Golden hamster)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of hamster IL10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 101 | |||||
| Range % | 91-105 | ||||||
| 1:2 | Average % | 106 | |||||
| Range % | 97-110 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 87-99 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 84-97 | ||||||
回收率：
| The recovery of hamster IL10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 93 | 88-97 | |||||
| EDTA plasma (n=4) | 95 | 90-101 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected4001.7481.7981.7731.5592001.3021.3431.3231.1091000.9390.9730.9560.742500.7100.6990.7050.491250.4880.5110.5000.28612.50.4090.4170.4130.1996.250.3110.3230.3170.10300.2120.2160.214 | |||||||
货期：3-5 working days
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