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产品描述：鱼胰岛素样生长因子II(IGF2)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的IGF2含量。IGF2即胰岛素样生长因子2，是一种多功能细胞增殖调控因子，在胚胎生长发育中起重要作用。其研究机制主要围绕与受体结合激活下游信号通路，影响细胞的增殖、分化和存活，异常表达与多种肿瘤的发生、发展密切相关。试剂盒检测范围为62.5 pg/mL-1000 pg/mL，本试剂盒适用于水产养殖研究、鱼类生理学实验、生长调控机制探究等科研领域，特别适用于评估饲料营养配比、环境胁迫对鱼类生长的影响或基因工程模型的生物标志物分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：igf2Insulin-like growth factor II ELISA Kit; IGF-II ELISA Kit; Erythrotropin ELISA Kit
缩写：IGF2
Uniprot No.：Q02816
种属：Fish
样本类型：serum, plasma
检测范围：62.5 pg/mL-1000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish IGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 91 | |||||
| Range % | 87-102 | ||||||
| 1:200 | Average % | 95 | |||||
| Range % | 91-99 | ||||||
| 1:400 | Average % | 96 | |||||
| Range % | 88-106 | ||||||
| 1:800 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
回收率：
| The recovery of fish IGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 90-102 | |||||
| EDTA plasma (n=4) | 93 | 85-102 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2Average 10000.0850.0890.087 5000.1920.2050.199 2500.4850.4890.487 1250.9420.9370.940 62.51.6131.5721.593 02.5952.6242.610 | |||||||
货期：3-5 working days
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