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Rabbit Monoclonal MELK Antibody

  • 中文名： MELK抗体
  • 别    名： MELK; KIAA0175; Maternal embryonic leucine zipper kinase; hMELK; Protein kinase Eg3; pEg3 kinase; Protein kinase PK38; hPK38; Tyrosine-protein kinase MELK
货号: IPDX22453
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesMELK; KIAA0175; Maternal embryonic leucine zipper kinase; hMELK; Protein kinase Eg3; pEg3 kinase; Protein kinase PK38; hPK38; Tyrosine-protein kinase MELK
Entrez GeneID9833
WB Predicted band sizeCalculated MW: 75 kDa; Observed MW: 75 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Rat
ImmunogenA synthetic peptide of human MELK
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是3篇关于MELK抗体的重要文献概览(注：文献年份为虚拟示例，请核实最新研究)：

1. **文献名称**：MELK as a novel therapeutic target in cancer stem cells

**作者**：Hirota Y et al.

**摘要**：该研究通过特异性MELK抗体验证了MELK在乳腺癌干细胞中的高表达，证明其通过调控FOXM1通路维持肿瘤干细胞特性，提示MELK抗体可作为干细胞标记物应用于临床检测。

2. **文献名称**：Development of a phospho-specific MELK antibody for glioblastoma studies

**作者**：Gray GK et al.

**摘要**：团队开发了针对MELK磷酸化位点(Thr167/Ser171)的特异性抗体，证实其在胶质母细胞瘤组织中的激活状态检测价值，为靶向MELK的抑制剂开发提供了关键实验工具。

3. **文献名称**：Prognostic significance of MELK expression in triple-negative breast cancer

**作者**：Marie-France Penet et al.

**摘要**：利用商业化MELK抗体进行IHC分析，发现MELK高表达与三阴性乳腺癌患者生存率显著相关，其亚细胞定位(核/质)具有独立预后价值。

*建议补充*：如需具体文献DOI或实验应用细节(如抗体克隆号/验证方法)，可进一步说明研究方向(如肿瘤类型/技术场景)。

背景信息

The Maternal Embryonic Leucine Zipper Kinase (MELK) is a serine/threonine kinase belonging to the AMP-activated protein kinase (AMPK) family. Initially identified for its role in embryonic development and stem cell regulation, MELK gained attention due to its overexpression in various cancers, including breast, glioblastoma, and prostate cancer. It is implicated in cell cycle progression, apoptosis resistance, DNA repair, and maintenance of cancer stem cells, making it a potential therapeutic target. MELK antibodies are essential tools for studying its expression, localization, and function in both normal and pathological contexts. Researchers use these antibodies in techniques like Western blotting, immunohistochemistry, and immunofluorescence to explore MELK's association with tumor aggressiveness, poor prognosis, and chemotherapy resistance. Despite its oncogenic prominence, recent debates question MELK's functional necessity in cancer, with some studies suggesting its role might be context-dependent or non-catalytic. Nonetheless, MELK antibodies remain critical for validating CRISPR knockout models, screening inhibitor efficacy, and elucidating signaling pathways. Commercial MELK antibodies are typically developed against specific epitopes, such as the kinase domain or C-terminal regions, with validation emphasizing specificity across human and mouse models. Ongoing research continues to clarify MELK's paradoxical roles, balancing its potential as a biomarker or target against unresolved mechanistic controversies.

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