| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAP2K2; MEK2; MKK2; PRKMK2; Dual specificity mitogen-activated protein kinase kinase 2; MAP kinase kinase 2; MAPKK 2; ERK activator kinase 2; MAPK/ERK kinase 2; MEK 2 |
| Entrez GeneID | 5605 |
| WB Predicted band size | Calculated MW: 44 kDa; Observed MW: 44 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic peptide of human MEK2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于MEK2抗体的示例参考文献(注：以下内容为示例，非真实文献)：
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1. **文献名称**: "Development and Characterization of a High-Specificity MEK2 Antibody for Signal Transduction Studies"
**作者**: Smith J, et al.
**摘要**: 本研究开发并验证了一种高特异性的MEK2多克隆抗体，能够选择性识别MEK2蛋白而非MEK1.通过Western blot和免疫荧光实验证明该抗体在多种细胞系中有效检测MEK2的表达水平及亚细胞定位，适用于MAPK/ERK信号通路研究。
2. **文献名称**: "MEK2-Specific Antibody Reveals Distinct Roles in Cancer Cell Proliferation"
**作者**: Lee H, et al.
**摘要**: 利用MEK2特异性单克隆抗体，研究发现MEK2在结直肠癌细胞中高表达且与ERK激活显著相关。抗体阻断实验表明抑制MEK2活性可降低肿瘤细胞的增殖和迁移能力，提示其作为治疗靶点的潜力。
3. **文献名称**: "Comparative Analysis of MEK1 and MEK2 Antibodies in Neurodegenerative Disease Models"
**作者**: Garcia R, et al.
**摘要**: 通过比较多种市售MEK1/MEK2抗体的交叉反应性，发现部分抗体存在非特异性结合问题。研究推荐使用经过验证的MEK2抗体(克隆号X7B)用于阿尔茨海默病模型中MAPK通路异常的检测。
4. **文献名称**: "MEK2 Inhibition via Antibody-Based Targeting in Drug-Resistant Melanoma"
**作者**: Patel S, et al.
**摘要**: 开发了一种针对MEK2的阻断性抗体，可有效抑制BRAF突变型黑色素瘤细胞的耐药性生长。实验表明该抗体通过阻断MEK2-ERK相互作用发挥疗效，为克服靶向治疗耐药性提供新策略。
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**建议**：实际研究中请通过PubMed、Web of Science等平台以“MEK2 antibody”、“MAP2K2 antibody”为关键词检索最新文献，并关注高影响因子期刊(如Nature, Cell, JBC)上的抗体验证研究。
MEK2 (Mitogen-activated protein kinase kinase 2), also known as MAP2K2. is a serine/threonine kinase within the MAPK/ERK signaling pathway, which regulates critical cellular processes such as proliferation, differentiation, and apoptosis. MEK2 functions as a dual-specificity kinase that phosphorylates and activates downstream ERK1/2 (Extracellular Signal-Regulated Kinases 1/2) upon stimulation by growth factors, cytokines, or mitogens. Dysregulation of MEK2 is implicated in various diseases, including cancers, inflammatory disorders, and developmental abnormalities.
MEK2 antibodies are essential tools for studying the expression, activation, and localization of MEK2 in biological systems. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to detect MEK2 protein levels or its phosphorylated (activated) form. Specific MEK2 antibodies can distinguish MEK2 from its closely related isoform MEK1. despite their high sequence homology. However, cross-reactivity with other family members should be validated experimentally. Phospho-specific MEK2 antibodies targeting conserved phosphorylation sites (e.g., Ser222/226) are critical for assessing pathway activation status in response to stimuli or therapeutic interventions.
Research applications include investigating MEK2's role in Ras-driven cancers, evaluating MEK inhibitor efficacy in precision medicine, and exploring signaling crosstalk in disease models. Reliable MEK2 antibodies thus underpin mechanistic studies of cellular signaling and drug development targeting the MAPK pathway.
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