关键信息
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基因名
ECD
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简介
ECD proteins are multifunctional regulators of cellular dynamics that cooperate with TXNIP to stabilize and regulate p53/TP53 by inhibiting MDM2-mediated degradation. In addition to p53 stabilization, ECD is involved in complex transcriptional regulation, exhibits intrinsic transactivation activity, and may interact with EP300. ECD Protein, Human (sf9, His-GST) is the recombinant human-derived ECD protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- 应用
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别名
Protein ecdysoneless homolog; hSGT1; ECD
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种属
Human
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表达系统
Baculovirus
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标签
N-His;N-GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O95905-1
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表达区间
M1-N644
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蛋白长度
Full Length of Isoform-1
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分子量
88-108 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
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