| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | XPNPEP2 |
| Uniprot No | O43895 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-649aa |
| 氨基酸序列 | HTKPVDLGG QDVRNCSTNP PYLPVTVVNT TMSLTALRQQ MQTQNLSAYI IPGTDAHMNE YIGQHDERRA WITGFTGSAG TAVVTMKKAA VWTDSRYWTQ AERQMDCNWE LHKEVGTTPI VTWLLTEIPA GGRVGFDPFL LSIDTWESYD LALQGSNRQL VSITTNLVDL VWGSERPPVP NQPIYALQEA FTGSTWQEKV SGVRSQMQKH QKVPTAVLLS ALEETAWLFN LRASDIPYNP FFYSYTLLTD SSIRLFANKS RFSSETLSYL NSSCTGPMCV QIEDYSQVRD SIQAYSLGDV RIWIGTSYTM YGIYEMIPKE KLVTDTYSPV MMTKAVKNSK EQALLKASHV RDAVAVIRYL VWLEKNVPKG TVDEFSGAEI VDKFRGEEQF SSGPSFETIS ASGLNAALAH YSPTKELNRK LSSDEMYLLD SGGQYWDGTT DITRTVHWGT PSAFQKEAYT RVLIGNIDLS RLIFPAATSG RMVEAFARRA LWDAGLNYGH GTGHGIGNFL CVHEWPVGFQ SNNIAMAKGM FTSIEPGYYK DGEFGIRLED VALVVEAKTK YPGSYLTFEV VSFVPYDRNL IDVSLLSPEH LQYLNRYYQT IREKVGPELQ RRQLLEEFEW LQQHTEPLA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于XPNPEP2重组蛋白的3篇代表性文献摘要(内容基于公开研究归纳，具体文献需通过学术数据库查询)：
1. **文献名称**：*"Expression and characterization of recombinant human X-prolyl aminopeptidase 2 (XPNPEP2) in Escherichia coli"*
**作者**：Smith A, et al.
**摘要**：该研究报道了人源XPNPEP2基因在大肠杆菌中的重组表达及纯化方法，通过优化诱导条件获得高纯度蛋白，并验证其催化降解X-Pro多肽的酶活性，为后续功能研究提供工具。
2. **文献名称**：*"Structural insights into substrate specificity of XPNPEP2 and its role in ACE inhibitor metabolism"*
**作者**：Johnson R, et al.
**摘要**：利用XPNPEP2重组蛋白进行晶体结构解析，揭示其底物结合域的特征，发现其对血管紧张素转换酶(ACE)抑制剂代谢产物(如缓激肽)的水解活性，提示其在药物代谢中的潜在作用。
3. **文献名称**：*"Genetic variation in XPNPEP2 influences response to angiotensin-converting enzyme inhibitors in patients with kidney disease"*
**作者**：Lee C, et al.
**摘要**：通过体外实验表达不同XPNPEP2基因突变体的重组蛋白，发现特定突变导致酶活性降低，与肾病患者对ACE抑制剂的治疗反应差异相关，提示XPNPEP2作为药物反应生物标志物的可能性。
**提示**：以上为模拟摘要，实际文献可通过PubMed、Google Scholar等平台以关键词“XPNPEP2 recombinant”或“XPNPEP2 expression”检索，并优先选择近5年发表的研究以获取最新进展。
**Background of XPNPEP2 Recombinant Protein**
XPNPEP2 (X-prolyl aminopeptidase 2), also known as membrane-bound prolyl carboxypeptidase (PRCP), is a zinc-dependent metalloprotease encoded by the *XPNPEP2* gene. It belongs to the M24B aminopeptidase family and plays a critical role in cleaving N-terminal proline residues from bioactive peptides, including bradykinin, angiotensin metabolites, and other regulatory substrates. This enzyme is predominantly localized to cell membranes and mitochondria, where it modulates peptide signaling pathways involved in blood pressure regulation, inflammation, and energy metabolism.
The recombinant XPNPEP2 protein is produced via genetic engineering in heterologous expression systems, such as *E. coli* or mammalian cell lines, to ensure high purity and bioactivity. Its recombinant form enables detailed biochemical studies, including substrate specificity, enzymatic kinetics, and inhibitor screening. Research has linked XPNPEP2 dysfunction to human diseases, such as hypertension, diabetic nephropathy, and autosomal recessive renal tubular dysfunction. Notably, mutations in *XPNPEP2* are associated with a rare genetic disorder causing hyperuricemia, renal stones, and progressive kidney failure.
In drug discovery, recombinant XPNPEP2 serves as a target for developing therapeutics targeting the renin-angiotensin and kallikrein-kinin systems. Additionally, it aids in biomarker studies for metabolic and cardiovascular disorders. Its role in degrading vasoactive peptides highlights its potential in modulating vascular tone and inflammation. Continued research on XPNPEP2 recombinant protein aims to unravel its physiological relevance and therapeutic applications in precision medicine.
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