首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人β胶原交联(bCTx)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的bCTx含量。试剂盒检测范围为125 pg/mL-2000 pg/mL，在科研场景中，本试剂盒可广泛应用于骨代谢机制研究、抗骨质疏松药物开发评估、骨骼疾病动物模型评价等领域，为骨转换相关的基础研究提供关键数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：bCTx
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：125 pg/mL-2000 pg/mL
灵敏度：125 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human bCTx in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 104 | ||||
| Range % | 96-108 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 83-92 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 93-104 | |||||
| 1:8 | Average % | 106 | ||||
| Range % | 100-110 | |||||
回收率：
| The recovery of human bCTx spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 82-97 | ||||
| EDTA plasma (n=4) | 93 | 87-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 20000.1590.1550.157 10000.3160.3010.309 5000.5030.4920.498 2500.9190.9360.928 1251.9381.8971.918 | ||||||
货期：3-5 working days
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