| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FAKTS; URLC9; hFLEG1 |
| Entrez GeneID | 55355 |
| WB Predicted band size | Calculated MW: 84 kDa; Observed MW: 84 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human HJURP |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于HJURP抗体的代表性文献(信息已简化整理)：
1. **文献名称**: "HJURP promotes hepatocellular carcinoma progression by modulating cell cycle checkpoint via CHK1"
**作者**: Li X, et al.
**摘要**: 研究利用HJURP抗体验证其在肝癌组织中的过表达，揭示其通过调控CHK1通路促进肝癌细胞周期进展和增殖的机制。
2. **文献名称**: "HJURP expression as a prognostic marker in triple-negative breast cancer"
**作者**: Wang Y, et al.
**摘要**: 通过免疫组化(HJURP抗体)发现HJURP在三阴性乳腺癌中高表达，且与患者不良预后相关，提示其作为潜在生物标志物的价值。
3. **文献名称**: "HJURP interacts with CENP-A chromatin to promote centromere integrity"
**作者**: Dunleavy EM, et al.
**摘要**: 利用HJURP抗体进行染色质免疫沉淀(ChIP)，证明HJURP直接结合CENP-A，维持着丝粒稳定性，对染色体分离至关重要。
4. **文献名称**: "Targeting HJURP inhibits tumor growth in pancreatic cancer models"
**作者**: Zhang L, et al.
**摘要**: 通过Western blot(HJURP抗体)和功能实验，证实抑制HJURP可阻碍胰腺癌细胞增殖，提示其作为治疗靶点的潜力。
(注：以上为模拟文献，实际引用请核对PubMed等数据库获取真实研究。)
HJURP (Holliday Junction Recognition Protein) is a key chaperone protein involved in the deposition of CENP-A, a centromere-specific histone H3 variant essential for proper kinetochore assembly and chromosome segregation during mitosis. Discovered in 2009. HJURP interacts directly with CENP-A and facilitates its incorporation into centromeric chromatin, ensuring epigenetic maintenance of centromere identity across cell divisions. Dysregulation of HJURP has been linked to chromosomal instability, mitotic errors, and cancer progression. Overexpression of HJURP is observed in multiple malignancies, including hepatocellular carcinoma, breast cancer, and gliomas, often correlating with poor prognosis.
HJURP-targeting antibodies are critical tools for studying centromere biology, DNA repair mechanisms, and cancer pathogenesis. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to detect HJURP expression levels, subcellular localization, and interaction partners. Commercial HJURP antibodies are typically raised against specific epitopes (e.g., human HJURP fragments aa 100-200 or aa 500-600) and validated for specificity through knockout cell lines or siRNA-mediated depletion. Recent research also explores HJURP inhibitors as potential anticancer therapies, highlighting the growing diagnostic and therapeutic relevance of HJURP-targeting reagents.
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