| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | hHIPk2; Hipk2; Nbak1; Stank |
| Entrez GeneID | 28996 |
| WB Predicted band size | Calculated MW: 131 kDa; Observed MW: 131 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human HIPK2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于HIPK2抗体的3篇参考文献示例(文献信息为示例，建议通过学术数据库核实准确性)：
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1. **文献名称**：*HIPK2 regulates p53 activity through direct phosphorylation and nucleo-cytoplasmic shuttling*
**作者**：D'Orazi, G., et al.
**摘要**：该研究利用HIPK2特异性抗体，揭示了HIPK2在DNA损伤后通过磷酸化p53并调控其核定位的机制，抗体用于免疫沉淀和免疫荧光实验，证实HIPK2在细胞应激反应中的关键作用。
2. **文献名称**：*Phosphorylation-dependent regulation of HIPK2 subcellular localization and kinase activity*
**作者**：Calzado, M.A., et al.
**摘要**：通过磷酸化特异性抗体，作者发现HIPK2的激活依赖其自身磷酸化状态，并证明其在细胞质与细胞核间的动态分布影响下游凋亡信号通路。
3. **文献名称**：*HIPK2-Myc interaction promotes tumor growth through transcriptional repression of pro-apoptotic genes*
**作者**：Pierantoni, G.M., et al.
**摘要**：研究使用HIPK2抗体进行免疫共沉淀和ChIP实验，发现HIPK2与Myc蛋白协同抑制促凋亡基因，促进癌症细胞存活，提示其在肿瘤发生中的潜在靶点价值。
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建议通过PubMed、Google Scholar等平台以“HIPK2 antibody”或“HIPK2 kinase function”为关键词检索最新文献，或结合具体实验需求(如WB、IHC应用)筛选。
HIPK2 (Homeodomain-Interacting Protein Kinase 2) is a serine/threonine kinase involved in critical cellular processes, including stress signaling, DNA damage response, apoptosis, and tumor suppression. It interacts with transcription factors (e.g., p53) and signaling pathways (e.g., Wnt, TGF-β) to regulate gene expression and cell fate decisions. HIPK2's activity is modulated by post-translational modifications and subcellular localization shifts, particularly under stress conditions. Dysregulation of HIPK2 has been linked to cancer, neurodegeneration, fibrosis, and diabetic complications, making it a research focus in disease mechanisms.
HIPK2 antibodies are essential tools for studying its expression, localization, and functional roles. These antibodies are used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect HIPK2 in human, mouse, or rat tissues and cell lines. Given HIPK2's nuclear-cytoplasmic shuttling under stress, reliable antibodies must recognize specific isoforms and phosphorylation states. Many commercial HIPK2 antibodies are validated using knockout controls or siRNA knockdown to ensure specificity. Researchers also employ them to explore HIPK2's interactions with partners like PML bodies or its role in pathways such as oxidative stress-induced apoptosis. However, variability in antibody performance across applications requires careful optimization in experimental settings.
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