| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EZR; VIL2; Ezrin; Cytovillin; Villin-2; p81 |
| Entrez GeneID | 7430 |
| WB Predicted band size | Calculated MW: 69 kDa; Observed MW: 80 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human Ezrin |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于Ezrin抗体的代表性文献，简要概括如下：
1. **《Ezrin promotes invasion and metastasis by interacting with MTA1 in hepatocellular carcinoma》**
- 作者：Li Q, et al.
- 摘要：研究揭示了Ezrin通过与转移相关蛋白MTA1的相互作用，激活下游信号通路，促进肝癌细胞的侵袭和转移，强调了Ezrin抗体在检测其表达水平及功能研究中的关键作用。
2. **《Ezrin modulates the cell surface expression of programmed death ligand-1 in human cancer cells》**
- 作者：Yao S, et al.
- 摘要：本文利用Ezrin特异性抗体，发现Ezrin通过调控PD-L1的膜定位影响肿瘤免疫逃逸，为靶向Ezrin-PD-L1轴的癌症免疫治疗提供了实验依据。
3. **《Ezrin is required for efficient apical endocytosis in polarized epithelial cells》**
- 作者：Casaletti L, et al.
- 摘要：通过Ezrin抗体的免疫荧光和功能阻断实验，研究证实Ezrin在极性上皮细胞的顶端膜内吞过程中起关键作用，其缺失导致细胞膜运输功能紊乱。
4. **《Ezrin mediates CD44-induced signaling in bladder cancer》**
- 作者：Khanna C, et al.
- 摘要：利用Ezrin抗体阻断实验，发现CD44通过激活Ezrin-Rho通路驱动膀胱癌迁移和化疗耐药，揭示了Ezrin作为治疗靶点的潜力。
以上文献均通过Ezrin抗体开展机制研究，涵盖癌症转移、免疫调控和细胞极性等方向，可作为相关领域的关键参考文献。
**Background of Ezrin Antibodies**
Ezrin, a member of the ERM (ezrin-radixin-moesin) protein family, acts as a critical linker between the plasma membrane and the actin cytoskeleton, regulating cell morphology, adhesion, and motility. It functions in diverse cellular processes, including signal transduction, membrane trafficking, and cell polarization. Structurally, ezrin comprises an N-terminal F-actin-binding domain and a C-terminal membrane-binding domain, connected by a helical region. Its activity is modulated by phosphorylation and interactions with signaling molecules like Rho GTPases.
Ezrin’s role in cancer metastasis and invasive diseases (e.g., breast cancer, melanoma, neuroblastoma) has driven interest in studying its expression and function. Overexpression or aberrant activation of ezrin correlates with enhanced tumor cell migration, invasion, and poor prognosis.
Ezrin antibodies are essential tools for detecting and quantifying ezrin in research and diagnostics. These antibodies, often monoclonal or polyclonal, are validated for techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). They help identify ezrin’s localization (e.g., membrane-cytoskeleton interface) and post-translational modifications. Specific clones or host-derived antibodies (e.g., mouse, rabbit) are selected based on application needs. Validation via knockout controls ensures specificity.
By enabling the study of ezrin’s molecular mechanisms and its association with disease progression, these antibodies contribute to exploring therapeutic targets or diagnostic biomarkers in oncology and cell biology research.
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