首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠白介素16(IL-16)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL16含量。IL16是一个重要靶点。它在免疫调节等生理过程中发挥关键作用。研究机制上，主要聚焦于其在细胞信号传导通路中的作用，以及与炎症、免疫相关疾病的关联，解析其如何影响细胞功能和疾病发展进程，为相关药物研发提供方向。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于小鼠疾病模型构建中的炎症因子动态监测、药物干预效果评估以及免疫调控机制的基础研究，为科研工作者研究IL - 16在免疫相关通路中的功能提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il16 ELISA Kit; Pro-interleukin-16 [Cleaved into: Interleukin-16 ELISA Kit; IL-16 ELISA Kit; Lymphocyte chemoattractant factor ELISA Kit; LCF)] ELISA Kit
缩写：IL16
Uniprot No.：O54824
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 80-99 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-101 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 92-100 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 86-115 | |||||
回收率：
| The recovery of mouse IL-16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 80-115 | ||||
| EDTA plasma (n=4) | 95 | 90-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.7502.8002.7752.6782502.0582.1482.1032.0061251.2581.3661.3121.21562.50.7850.8400.8130.71631.20.5200.4820.5010.40415.60.3850.3830.3840.2877.80.1960.1990.1980.10100.0980.0960.097 | ||||||
货期：3-5 working days
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