| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | BCDIN3D |
| Uniprot No | Q7Z5W3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-292aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAVPTEL DGGSVKETAA EEESRVLAPG AAPFGNFPHY SRFHPPEQRL RLLPPELLRQ LFPESPENGP ILGLDVGCNS GDLSVALYKH FLSLPDGETC SDASREFRLL CCDIDPVLVK RAEKECPFPD ALTFITLDFM NQRTRKVLLSS FLSQFGRSV FDIGFCMSIT MWIHLNHGDH GLWEFLAHLS SLCHYLLVEP QPWKCYRAAA RRLRKLGLHD FDHFHSLAIR GDMPNQIVQI LTQDHGMELI CCFGNTSWDR SLLLFRAKQT IETHPIPESL IEKGKEKNRL SFQKQ |
| 预测分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BCDIN3D重组蛋白的3篇代表性文献概览(注：文献信息为模拟示例，实际引用需核实原始来源)：
1. **文献名称**: *Structural and biochemical characterization of the human RNA methyltransferase BCDIN3D*
**作者**: Cloutier, P., et al.
**摘要**: 通过重组蛋白技术解析BCDIN3D的晶体结构，揭示其作为甲基转移酶的活性位点及底物特异性，证实其对5'-单磷酸RNA的甲基化修饰功能。
2. **文献名称**: *BCDIN3D regulates miR-145 maturation by pri-miRNA methylation*
**作者**: Suzuki, H., et al.
**摘要**: 研究利用重组BCDIN3D蛋白，证明其通过甲基化pri-miR-145影响miRNA加工，进而抑制乳腺癌细胞侵袭，揭示其表观遗传调控机制。
3. **文献名称**: *BCDIN3D is a regulator of glucose metabolism in triple-negative breast cancer*
**作者**: Alarcón, C.R., et al.
**摘要**: 通过重组蛋白功能实验，发现BCDIN3D通过调控代谢相关RNA的甲基化，促进三阴性乳腺癌细胞的糖酵解通路，提示其作为潜在治疗靶点。
4. **文献名称**: *Enzymatic characterization of recombinant BCDIN3D and its role in tRNA modification*
**作者**: Dias, J., et al.
**摘要**: 利用大肠杆菌表达系统获得重组BCDIN3D蛋白，验证其甲基转移酶活性及对特定tRNA的修饰作用，拓展其在RNA代谢中的生物学意义。
(注：以上文献信息为基于领域知识的模拟生成，实际引用请以真实发表文献为准。)
The BCDIN3D protein, encoded by the BCDIN3D gene, is a relatively understudied RNA methyltransferase implicated in post-transcriptional gene regulation. It belongs to the Bin3/MTD1 methyltransferase superfamily, characterized by a conserved S-adenosyl-L-methionine (SAM)-binding domain. BCDIN3D catalyzes the methylation of 5'-monophosphate groups in specific non-coding RNAs, particularly primary microRNAs (pri-miRNAs), through its unique 5'-monophosphate methyltransferase activity.
First identified as a phosphoprotein interacting with the Bicoid homeodomain, BCDIN3D gained attention for its role in modulating miRNA maturation. It methylates the 5' end of pri-miR-145 and pri-miR-21. influencing their processing by Drosha/DGCR8 complexes and subsequent miRNA functionality. This methylation appears to fine-tune miRNA-mediated gene silencing, particularly in pathways regulating cell proliferation and differentiation.
Emerging studies link BCDIN3D to cancer biology, with overexpression observed in triple-negative breast cancer and other malignancies. Its enzymatic activity enhances tumor cell migration and invasion, potentially through dysregulation of metastasis-associated miRNAs. The protein’s involvement in insulin signaling and metabolic pathways has also been reported, suggesting broader regulatory functions beyond miRNA processing.
Recombinant BCDIN3D proteins are typically expressed in E. coli or mammalian systems with affinity tags for purification. These tools enable structural studies of its unique substrate recognition mechanism and high-throughput screening for potential inhibitors. Current research focuses on delineating its physiological substrates, tissue-specific roles, and therapeutic potential as a cancer target. Despite progress, the full spectrum of its biological functions and molecular partners remains to be elucidated.
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