首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胰抑制素酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的含量。人胰抑制素是一种由胰岛β细胞分泌的肽类激素，与胰岛素协同调节血糖稳态。其研究显示，人胰抑制素能延缓胃排空、降低餐后血糖，并通过调节胰岛素和胰高血糖素的分泌来维持血糖平衡。其作用机制涉及影响胰岛素分泌、调节血糖和脂肪代谢等方面。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，科研人员可将其应用于探究在糖尿病、肥胖症等代谢异常病理机制中的作用，或用于评估药物干预、基因编辑等实验模型中该蛋白的动态表达水平变化，为相关基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Pancreastatin
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human pancreastatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 100 | ||||
| Range % | 95-110 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human pancreastatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-99 | ||||
| EDTA plasma (n=4) | 90 | 85-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.4392.3522.3962.26710001.8081.6761.7421.6135001.2191.1841.2021.0732500.7040.7390.7220.5931250.4130.4240.4190.29062.50.3170.3290.3230.19431.250.2340.2290.2320.10300.1310.1270.129 | ||||||
货期：3-5 working days
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