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产品描述：人胰多肽(PP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的PP含量。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于基础科研中探索PP在代谢调控、内分泌信号通路中的作用机制，或用于评估药物干预、病理模型下PP的动态变化，可满足体外实验、动物模型或细胞培养体系中PP表达水平的定量分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：PPY ELISA Kit; PNPPancreatic prohormone ELISA Kit; Pancreatic polypeptide ELISA Kit; PP ELISA Kit; Obinepitide) [Cleaved into: Pancreatic hormone ELISA Kit; PH); Pancreatic icosapeptide ELISA Kit; PI)] ELISA Kit
缩写：PP
Uniprot No.：P01298
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 83-89 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 84-102 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 94-100 | |||||
回收率：
| The recovery of human PP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 95-94 | ||||
| EDTA plasma (n=4) | 108 | 104-112 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.3532.3692.3612.23910001.4701.4201.4451.3235000.7950.7630.7790.6572500.4730.4980.4860.3641250.3150.3280.3220.20062.50.2320.2410.2370.11531.250.1710.1790.1750.05300.1230.1200.122 | ||||||
货期：3-5 working days
产品描述：人胰多肽(PP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的PP含量。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于基础科研中探索PP在代谢调控、内分泌信号通路中的作用机制，或用于评估药物干预、病理模型下PP的动态变化，可满足体外实验、动物模型或细胞培养体系中PP表达水平的定量分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：PPY ELISA Kit; PNPPancreatic prohormone ELISA Kit; Pancreatic polypeptide ELISA Kit; PP ELISA Kit; Obinepitide) [Cleaved into: Pancreatic hormone ELISA Kit; PH); Pancreatic icosapeptide ELISA Kit; PI)] ELISA Kit
缩写：PP
Uniprot No.：P01298
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 83-89 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 84-102 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 94-100 | |||||
回收率：
| The recovery of human PP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 95-94 | ||||
| EDTA plasma (n=4) | 108 | 104-112 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.3532.3692.3612.23910001.4701.4201.4451.3235000.7950.7630.7790.6572500.4730.4980.4860.3641250.3150.3280.3220.20062.50.2320.2410.2370.11531.250.1710.1790.1750.05300.1230.1200.122 | ||||||
货期：3-5 working days
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