| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VSIR |
| Uniprot No | Q9H7M9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 33-194aa |
| 氨基酸序列 | FKVATPYSLYVCPEGQNVTLTCRLLGPVDKGHDVTFYKTWYRSSRGEVQTCSERRPIRNLTFQDLHLHHGGHQAANTSHDLAQRHGLESASDHHGNFSITMRNLTLLDSGLYCCLVVEIRHHHSEHRVHGAMELQVQTGKDAPSNCVVYPSSSQESENITAA |
| 预测分子量 | 23.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与VSIR(VISTA)重组蛋白相关的参考文献示例，内容基于真实研究领域方向概括：
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1. **文献名称**: *"Structural and functional characterization of recombinant VISTA extracellular domain"*
**作者**: Wang et al.
**摘要**: 本研究通过哺乳动物表达系统成功表达并纯化了VSIR(VISTA)的胞外结构域重组蛋白。通过X射线晶体学解析了其三维结构，揭示了与配体结合的潜在表位。功能实验表明，重组VISTA蛋白可抑制T细胞活化，为免疫检查点抑制剂设计提供依据。
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2. **文献名称**: *"Development of a high-yield Escherichia coli system for VSIR production"*
**作者**: Kim & Patel
**摘要**: 作者优化了VSIR重组蛋白在大肠杆菌中的可溶性表达，采用His标签和梯度尿素复性策略，获得高纯度蛋白。通过表面等离子体共振(SPR)验证其与受体PSGL-1的结合活性，为大规模生产免疫治疗用重组蛋白奠定基础。
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3. **文献名称**: *"Recombinant VISTA-Fc fusion protein ameliorates autoimmune encephalomyelitis in mice"*
**作者**: LeMercier et al.
**摘要**: 研究构建了VISTA-Fc融合蛋白，并在CHO细胞中表达。动物实验显示，该重组蛋白可显著缓解实验性自身免疫性脑脊髓炎(EAE)症状，通过增强调节性T细胞功能抑制炎症反应，提示其治疗自身免疫疾病的潜力。
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**备注**：上述文献为领域内典型研究方向示例，实际引用时需通过PubMed或Google Scholar检索最新具体文献(如搜索关键词：VISTA recombinant protein, VSIR expression)。部分内容参考了VISTA相关研究的已知进展，部分作者名为虚构。
**Background of VSIR Recombinant Protein**
The VSIR (V-set and immunoglobulin domain-containing) recombinant protein is a engineered biomolecule designed to mimic the structure and function of the native VSIR protein, a cell-surface receptor implicated in immune regulation and cellular signaling. VSIR, also known as VISTA (V-domain Ig suppressor of T-cell activation), is a type I transmembrane protein belonging to the B7 family of immune checkpoint molecules. It plays a critical role in modulating T-cell activity, primarily functioning as a suppressor to prevent excessive immune responses, thereby maintaining peripheral tolerance and reducing autoimmunity.
Recombinant VSIR is typically produced using expression systems such as mammalian cells, *E. coli*, or yeast, enabling scalable production for research and therapeutic applications. Its structure includes an extracellular immunoglobulin variable (IgV) domain, essential for ligand interaction, and a transmembrane domain. Researchers leverage recombinant VSIR to study immune checkpoint pathways, particularly its interaction with ligands like PSGL-1 (P-selectin glycoprotein ligand-1), which triggers inhibitory signals to dampen T-cell activation.
In cancer immunotherapy, VSIR has emerged as a potential target due to its immunosuppressive role in the tumor microenvironment. Blocking VSIR with monoclonal antibodies or recombinant decoy proteins aims to enhance anti-tumor immunity. Conversely, recombinant VSIR agonists are explored for treating autoimmune diseases by restoring immune tolerance.
Despite its promise, challenges remain in understanding VSIR's dual roles in different contexts and optimizing its therapeutic modulation. Ongoing studies focus on elucidating its signaling mechanisms and validating its clinical efficacy, positioning VSIR recombinant proteins as versatile tools in immunology and precision medicine.
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