首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人内皮素1(ET-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织培养上清液、尿液、唾液样本中的EDN1含量。EDN1即内皮素-1，是一种具有强烈而持久的缩血管效应以及促进细胞增殖和肥大作用的内源性活性肽。其研究机制主要聚焦于它参与调节血管张力、细胞增殖等生理过程，在心血管疾病、肿瘤等多种疾病病理发展中可能起重要作用。试剂盒检测范围为3.12 pg/mL-200 pg/mL，本试剂盒适用于心血管疾病机制研究、药物开发中ET - 1调节剂的效果评估、体液生物标志物探索等科研领域，尤其支持跨样本类型的动态监测，例如体外细胞模型分泌水平分析、体液样本中ET - 1浓度变化研究等，为心血管及代谢相关疾病的分子机制解析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ARCND3 ELISA Kit; Big endothelin-1 ELISA Kit; EDN1 ELISA Kit; EDN1_HUMAN ELISA Kit; ET-1 ELISA Kit; ET1 ELISA Kit; HDLCQ7 ELISA Kit; PPET1 ELISA Kit; Preproendothelin 1 ELISA Kit; Preproendothelin-1 ELISA Kit; QME ELISA Kit
缩写：EDN1
Uniprot No.：P05305
种属：Homo sapiens (Human)
样本类型：serum, cell culture supernates, urine, saliva
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 82-97 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 94-103 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 90-105 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 82-91 | |||||
回收率：
| The recovery of human ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.7222.7522.7372.6281002.1692.3222.2462.137501.5451.5281.5371.428250.8820.9010.8920.78312.50.5510.5750.5630.4546.250.3410.3510.3460.2373.120.2450.2450.2450.13600.1120.1050.109 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ET-1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled ET-1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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