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Human Follistatin Like Protein 1(FSTL1) ELISA Kit

  • 中文名： 人卵泡抑素样蛋白1(FSTL-1)ELISA Kit
货号: IPD-E13516h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人卵泡抑素样蛋白1（FSTL-1）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FSTL1含量。FSTL1（卵泡抑素样蛋白1）是一种在多种纤维化疾病中发挥关键作用的分泌性糖蛋白。研究显示，FSTL1通过促进TGF-β1/Smad3信号通路介导的皮肤成纤维细胞活化，加剧皮肤纤维化进程。此外，FSTL1在肝癌转移和干细胞分化中也扮演重要角色，其表达与肿瘤的进展和预后密切相关。试剂盒检测范围为0.156 ng/ml-10 ng/ml，通过优化的实验体系，可在科研实验中用于探索FSTL - 1在疾病模型中的表达调控机制、评估药物干预效果或解析其在细胞信号通路中的作用，为心血管、免疫及再生医学等领域的基础研究提供可靠工具。试剂盒操作便捷，配套标准品及缓冲液，适用于实验室常规检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Anti FSTL1 ELISA Kit; FLJ50214 ELISA Kit; FLJ52277 ELISA Kit; Follistatin like 1 ELISA Kit; Follistatin related protein ELISA Kit; Follistatin-like protein 1 ELISA Kit; Follistatin-related protein 1 ELISA Kit; FRP ELISA Kit; FSL1 ELISA Kit; FSTL1 ELISA Kit; FSTL1 ELISA Kit ELISA Kit; FSTL1_HUMAN ELISA Kit; Micro RNA 198 ELISA Kit; MIR198 ELISA Kit; miRNA198 included ELISA Kit; OCC 1 ELISA Kit; OCC1 ELISA Kit
  • 缩写：
    FSTL1
  • Uniprot No.：
    Q12841
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/ml -10 ng/ml
  • 灵敏度：
    0.039 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human FSTL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    101


    Range %

    94-107


    1:2

    Average %

    93


    Range %

    89-98


    1:4

    Average %

    95


    Range %

    88-101


    1:8

    Average %

    89


    Range %

    85-95


  • 回收率：

    The recovery of human FSTL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    88-101


    EDTA plasma (n=4)

    102

    95-108


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    10

    2.008

    2.034

    2.021

    1.830


    5

    1.264

    1.247

    1.256

    1.064


    2.5

    0.817

    0.838

    0.828

    0.636


    1.25

    0.528

    0.509

    0.519

    0.327


    0.625

    0.385

    0.371

    0.378

    0.187


    0.312

    0.328

    0.313

    0.321

    0.129


    0.156

    0.270

    0.284

    0.277

    0.086


    0

    0.188

    0.195

    0.192




  • 货期：
    3-5 working days

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