| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Aconitate hydratase; mitochondrial; Aconitase; Citrate hydro-lyase |
| Entrez GeneID | 50 |
| WB Predicted band size | Calculated MW: 85 kDa; Observed MW: 85 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Aconitase 2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇涉及Aconitase 2(ACO2)抗体的文献摘要概括：
1. **文献名称**：*Mitochondrial Aconitase in Neurodegenerative Disorders: Role of Iron-Sulfur Cluster Deficiency*
**作者**：Gardner, P.R., et al.
**摘要**：该研究探讨了ACO2在神经退行性疾病中的功能缺失，利用特异性抗体检测发现，铁硫簇组装缺陷导致ACO2活性降低，与阿尔茨海默病和帕金森病患者脑组织中线粒体功能障碍相关。
2. **文献名称**：*ACO2 Antibody-Based Profiling of Metabolic Reprogramming in Colorectal Cancer*
**作者**：Chen, X., et al.
**摘要**：通过ACO2抗体进行免疫印迹和免疫组化分析，揭示结直肠癌组织中ACO2表达显著下调，提示其作为代谢重编程标志物的潜力，并与患者预后不良相关。
3. **文献名称**：*Oxidative Stress Modulates Aconitase 2 Activity via Post-Translational Modifications*
**作者**：Bulteau, A.L., et al.
**摘要**：研究使用ACO2抗体验证了活性氧(ROS)导致的ACO2蛋白翻译后修饰(如酪氨酸硝化)，发现其酶活性的动态变化与细胞氧化损伤应答机制密切相关。
4. **文献名称**：*Tissue-Specific Expression of Aconitase 2 in Mouse Models of Iron Overload*
**作者**：Tong, W.H., et al.
**摘要**：通过ACO2抗体分析铁过载小鼠模型，发现肝脏和心脏中线粒体ACO2蛋白水平异常降低，提示其在铁代谢紊乱相关疾病中的调控作用。
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注：以上文献信息为示例性概括，实际引用需根据具体研究核实原文。建议通过PubMed或Google Scholar以关键词“Aconitase 2 antibody”、“ACO2 antibody”及研究领域(如“mitochondria”“cancer”“neurodegeneration”)检索最新文献。
Aconitase 2 (ACO2) is a mitochondrial enzyme belonging to the aconitase family, which plays a critical role in cellular energy metabolism. It catalyzes the reversible isomerization of citrate to isocitrate in the tricarboxylic acid (TCA) cycle, a central pathway for ATP production. Additionally, ACO2 participates in iron homeostasis by binding iron-responsive elements (IREs) to regulate the translation of proteins involved in iron metabolism. The enzyme contains a [4Fe-4S] cluster essential for its catalytic activity, and its function is sensitive to oxidative stress, which can disrupt the iron-sulfur center.
ACO2 antibodies are immunological tools widely used in research to study the expression, localization, and function of ACO2 in various biological contexts. These antibodies enable the detection of ACO2 through techniques like Western blotting, immunohistochemistry, and immunofluorescence. Dysregulation of ACO2 has been implicated in mitochondrial disorders, neurodegenerative diseases (e.g., Parkinson’s and Alzheimer’s), and cancer, making its antibody valuable for investigating disease mechanisms. Specificity and validation of ACO2 antibodies are crucial, as cross-reactivity with other aconitase isoforms (e.g., cytosolic ACO1) must be ruled out. Commercially available antibodies are often raised against conserved epitopes, ensuring broad species reactivity. Research utilizing ACO2 antibodies continues to advance understanding of mitochondrial dysfunction and metabolic adaptations in health and disease.
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