首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胱天蛋白酶3(Casp-3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、细胞裂解物、组织匀浆样本中的CASP3含量。CASP3 即半胱氨酸天冬氨酸蛋白酶 3，它是细胞凋亡过程中的关键执行分子。在细胞凋亡信号转导中发挥核心作用，能切割众多底物蛋白，促使细胞呈现凋亡特征。对其研究机制的深入探索，有助于理解疾病发病机制，为开发相关治疗药物提供靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于体外药物开发评估、凋亡通路机制研究、疾病模型样本检测等科研场景，例如通过检测细胞或组织样本中Caspase - 3的表达变化，探究凋亡诱导剂或抑制剂的效应，或解析特定病理条件下细胞死亡调控机制。适用于实验室常规蛋白定量研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：A830040C14Rik ELISA Kit; Apopain ELISA Kit; CASP 3 ELISA Kit; CASP-3 ELISA Kit; CASP3 ELISA Kit; CASP3_HUMAN ELISA Kit; Casp3a ELISA Kit; Caspase 3 ELISA Kit; Caspase 3; apoptosis-related cysteine peptidase ELISA Kit; Caspase 3; apoptosis-related cysteine protease ELISA Kit; Caspase 3; apoptosis-related cysteine protease a ELISA Kit; Caspase-3 subunit p12 ELISA Kit; Caspase3 ELISA Kit; CC3 ELISA Kit; CPP 32 ELISA Kit; CPP-32 ELISA Kit; CPP32 ELISA Kit; CPP32B ELISA Kit; Cysteine protease CPP32 ELISA Kit; EC 3.4.22.56 ELISA Kit; ICE3 ELISA Kit; LICE ELISA Kit; mldy ELISA Kit; OTTHUMP00000165052 ELISA Kit; OTTHUMP00000165053 ELISA Kit; OTTHUMP00000165054 ELISA Kit; PARP cleavage protease ELISA Kit; Procaspase3 ELISA Kit; Protein Yama ELISA Kit; SCA 1 ELISA Kit; SCA-1 ELISA Kit; SCA1 ELISA Kit; SREBP cleavage activity 1 ELISA Kit; Yama ELISA Kit; Yama protein ELISA Kit
缩写：CASP3
Uniprot No.：P42574
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell lysates, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Casp-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-101 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 83-101 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 90-106 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 85-105 | |||||
回收率：
| The recovery of human Casp-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-104 | ||||
| EDTA plasma (n=4) | 96 | 91-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.7582.7622.7602.653 102.4762.4612.4692.362 52.0021.9021.9521.845 2.51.2451.2091.2271.120 1.250.6220.6120.6170.510 0.6250.3080.3250.3170.210 0.3120.2120.2240.2180.111 00.1050.1090.107 | ||||||
货期：3-5 working days
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