首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人骨成型蛋白15(BMP-15/GDF-9B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的BMP15含量。BMP15即骨形态发生蛋白 15，是卵母细胞分泌的生长因子，属于 TGF-β超家族成员。其在卵泡发育、卵巢功能维持等方面意义重大。研究多聚焦于它对颗粒细胞增殖分化、卵泡成熟及排卵的调控机制，以了解其在生殖生理及疾病中的作用。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于生殖生物学基础研究、卵巢功能评估、药物作用机制探索等领域，特别适用于体外培养模型中BMP - 15分泌水平的动态监测，为生殖内分泌调控、辅助生殖技术优化等科研方向提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BMP 15 ELISA Kit; BMP-15 ELISA Kit; BMP15 ELISA Kit; BMP15_HUMAN ELISA Kit; Bone morphogenetic protein 15 ELISA Kit; GDF 9B ELISA Kit; GDF-9B ELISA Kit; GDF9B ELISA Kit; Growth/differentiation factor 9B ELISA Kit; ODG2 ELISA Kit; POF4 ELISA Kit
缩写：BMP15
Uniprot No.：O95972
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BMP-15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-103 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 87-98 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 91-102 | |||||
回收率：
| The recovery of human BMP-15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 96-107 | ||||
| EDTA plasma (n=4) | 92 | 86-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.7652.6372.7012.5195002.2452.1782.2122.0302501.3761.4831.4301.2481250.7560.7840.7700.58862.50.4670.4810.4740.29231.20.3350.3210.3280.14615.60.2350.2450.2400.05800.1830.1810.182 | ||||||
货期：3-5 working days
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