| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NOTCH4; INT3; Neurogenic locus notch homolog protein 4; Notch 4; hNotch4 |
| Entrez GeneID | 4855 |
| WB Predicted band size | Calculated MW: 210 kDa; Observed MW: 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human NOTCH4 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是3-4篇关于Notch4抗体的参考文献及其摘要概括：
1. **"Notch4 Signaling in Tumor Angiogenesis"**
- **作者**: Smith A, et al.
- **摘要**: 研究探讨Notch4受体在肿瘤血管生成中的作用，利用特异性抗体检测其在肿瘤内皮细胞的表达，揭示其通过调控VEGF通路促进血管异常生长的机制。
2. **"Targeting Notch4 with Monoclonal Antibodies Suppresses Breast Cancer Progression"**
- **作者**: Chen L, et al.
- **摘要**: 开发了一种人源化抗Notch4单克隆抗体，通过抑制配体结合阻断Notch4信号传导，显著抑制三阴性乳腺癌细胞的迁移和侵袭，提示其治疗潜力。
3. **"Structural Characterization of Notch4 Extracellular Domain Using Antibody Mapping"**
- **作者**: Patel R, et al.
- **摘要**: 通过多克隆抗体对Notch4胞外结构域进行表位定位，解析其关键功能区域，为设计靶向Notch4的抑制剂提供结构基础。
4. **"Notch4 Expression in Pulmonary Hypertension: Insights from Immunohistochemical Analysis"**
- **作者**: Gonzalez M, et al.
- **摘要**: 使用抗Notch4抗体分析肺高压患者组织样本，发现Notch4在肺动脉平滑肌细胞中异常高表达，与疾病严重程度呈正相关。
(注：以上为模拟文献，实际引用时需核实真实存在的论文。)
Notch4 is a transmembrane receptor belonging to the Notch family (Notch1-4), which plays a critical role in cell-cell communication, development, and tissue homeostasis. The Notch signaling pathway is evolutionarily conserved and regulates processes like cell differentiation, proliferation, and apoptosis. Notch4. encoded by the *NOTCH4* gene in humans, shares structural similarities with other Notch receptors, including extracellular epidermal growth factor (EGF)-like repeats, a transmembrane domain, and an intracellular domain responsible for transcriptional regulation. However, Notch4 exhibits distinct expression patterns and functions, particularly in vascular development and endothelial cell behavior. Dysregulation of Notch4 has been implicated in diseases such as cancer, where it may promote tumor angiogenesis, metastasis, and chemoresistance.
Notch4 antibodies are essential tools for detecting and studying the receptor’s expression, activation, and localization in biological samples. These antibodies target specific epitopes, such as the extracellular domain (ECD) or intracellular domain (NICD), enabling applications like Western blotting, immunohistochemistry (IHC), flow cytometry, and immunofluorescence. Researchers use Notch4 antibodies to investigate its role in pathological conditions, including breast cancer, glioblastoma, and inflammatory disorders. Some antibodies distinguish between the full-length receptor and its cleaved, active form (NICD), aiding in mechanistic studies of Notch4 signaling.
Commercial Notch4 antibodies vary in specificity, host species, and clonality (monoclonal/polyclonal). Validation across experimental models is crucial due to potential cross-reactivity with other Notch family members. Understanding Notch4’s context-dependent roles through these antibodies contributes to therapeutic development, particularly for targeting Notch pathway aberrations in disease.
×
