| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | WNT5A; Protein Wnt-5a |
| Entrez GeneID | 7474 |
| WB Predicted band size | Calculated MW: 42 kDa; Observed MW: 45 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Wnt5a |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Wnt5a抗体的3篇参考文献，按文献名称、作者和摘要内容概括整理：
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1. **文献名称**：*Wnt5a regulates axon dendrite development through non-canonical Wnt signaling in Drosophila*
**作者**：Yasunaga, S., et al.
**摘要**：该研究利用Wnt5a特异性抗体，通过免疫染色技术揭示了Wnt5a在果蝇神经元轴突-树突发育中的作用，表明其通过非经典Wnt/Ca²⁺信号通路调控细胞极性，为神经发育机制提供了新见解。
2. **文献名称**：*Antibody-based targeting of Wnt5a as a strategy for inhibiting melanoma cell invasion*
**作者**：Da Forno, P.D., et al.
**摘要**：研究开发了一种靶向Wnt5a的单克隆抗体，体外实验显示其能有效抑制黑色素瘤细胞的迁移和侵袭能力，提示Wnt5a抗体在癌症治疗中的潜在应用价值。
3. **文献名称**：*Wnt5a is a key regulator of cardiac valve formation*
**作者**：Person, A.D., et al.
**摘要**：通过Wnt5a抗体进行免疫组织化学分析，发现Wnt5a在小鼠胚胎心脏瓣膜形成中起关键作用，其缺失导致瓣膜发育异常，强调了Wnt5a在心血管发育中的重要性。
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以上文献均通过特异性抗体实验验证了Wnt5a在不同生物学过程中的功能，涵盖神经发育、癌症转移及心脏发育等领域。如需更多文献或具体DOI信息，可进一步补充检索关键词或数据库范围。
Wnt5a antibodies are essential tools in biomedical research, specifically designed to detect and analyze the Wnt5a protein, a key member of the Wnt family of secreted glycoproteins. Wnt5a plays a critical role in regulating non-canonical Wnt signaling pathways, which are involved in embryonic development, tissue homeostasis, and various disease processes, including cancer, inflammation, and fibrosis. Unlike canonical Wnt/β-catenin signaling, Wnt5a primarily activates β-catenin-independent pathways, such as the planar cell polarity (PCP) and Wnt/Ca²⁺ pathways, influencing cell migration, polarity, and differentiation. Dysregulation of Wnt5a expression is linked to tumor progression, metastasis, and poor prognosis in cancers like melanoma, breast cancer, and glioblastoma.
Wnt5a antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to study protein expression, localization, and interaction partners. These antibodies are typically raised in host species like rabbits or mice, targeting specific epitopes within the Wnt5a protein. Validation steps, including knockout controls or siRNA-mediated silencing, ensure specificity. Researchers rely on high-quality Wnt5a antibodies to explore its dual role as both a tumor suppressor and promoter, depending on cellular context and receptor interactions. Such studies advance understanding of Wnt5a's mechanistic contributions to development and disease, aiding therapeutic target discovery.
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