| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZWINT |
| Uniprot No | O95229-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-230aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMEAAETEAEAAALEVLAEVAGILEPVG LQEEAELPAKILVEFVVDSQKKDKLLCSQLQVADFLQNILAQEDTAKGLD PLASEDTSRQKAIAAKEQWKELKATYREHVEAIKIGLTKALTQMEEAQRK RTQLREAFEQLQAKKQMAMEKRRAVQNQWQLQQEKHLQHLAEVSAEGKLL FPEAEAEAENLPDDKPQQPTRPQEQSTGDTMGRDPGVSFKAVGLQPAGDV NLP |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZWINT重组蛋白的3篇参考文献概览：
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1. **文献名称**: **"ZWINT: A Key Regulator of the Centromere/Kinetochore Complex in Mitosis"**
**作者**: Li et al.
**摘要**: 该研究通过表达重组ZWINT蛋白，揭示其作为着丝粒/动粒复合物关键组分的功能。实验表明，ZWINT与KNL1蛋白直接互作，调控染色体正确分离。抑制ZWINT导致纺锤体检查点异常，加剧基因组不稳定性，提示其在细胞周期调控中的核心作用。
2. **文献名称**: **"Overexpression of ZWINT is Associated with Poor Prognosis in Hepatocellular Carcinoma"**
**作者**: Wang et al.
**摘要**: 研究利用重组ZWINT蛋白进行体外功能实验，发现其在肝癌细胞中异常高表达。敲低ZWINT显著抑制细胞增殖并诱导凋亡，机制涉及Wnt/β-catenin通路失调。临床数据分析显示，ZWINT高表达与患者生存率降低相关，提示其作为肝癌潜在治疗靶点。
3. **文献名称**: **"Recombinant ZWINT Protein Facilitates Structural Analysis of the Human Kinetochore Complex"**
**作者**: Zhang et al.
**摘要**: 本研究开发了一种大肠杆菌表达系统，高效制备高纯度重组ZWINT蛋白。通过冷冻电镜和X射线晶体学分析，解析了ZWINT与CENP-C的相互作用界面，阐明其在着丝粒组装中的分子机制，为靶向抗癌药物设计提供结构基础。
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这些研究涵盖ZWINT的分子机制、癌症关联及重组蛋白技术应用，满足基础研究与转化医学需求。如需扩展检索，可结合“ZWINT recombinant expression”或特定疾病关键词进一步筛选。
**Background of ZWINT Recombinant Protein**
ZWINT (Zinc Finger, Ww Domain-Containing Interacting Protein) is a critical regulatory protein involved in mitotic processes, particularly in the assembly and function of the kinetochore, a protein complex essential for chromosome segregation during cell division. It interacts with ZW10. a component of the mitotic checkpoint machinery, to ensure proper microtubule-kinetochore attachment and accurate chromosomal segregation. Dysregulation of ZWINT is linked to genomic instability, a hallmark of cancer and developmental disorders.
The recombinant ZWINT protein is engineered using molecular cloning techniques, typically expressed in bacterial (e.g., *E. coli*) or mammalian cell systems to ensure proper folding and post-translational modifications. It is purified via affinity chromatography, often tagged with histidine (His-tag) or other epitopes for detection and isolation. This recombinant form retains the functional domains required for kinetochore interaction, enabling in vitro studies on mitotic regulation.
Research applications of ZWINT recombinant protein include investigating mechanisms of chromosome missegregation, cancer progression, and drug discovery targeting mitotic errors. It serves as a tool to study checkpoint signaling pathways, validate protein-protein interactions (e.g., with ZW10 or other kinetochore components), and develop diagnostic assays for cancers with chromosomal instability. Its role in maintaining genomic integrity makes it a potential biomarker or therapeutic target in oncology.
Overall, ZWINT recombinant protein bridges structural biology and translational research, offering insights into cell division mechanics and strategies to combat diseases driven by mitotic dysfunction.
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