| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BIRC5; API4; IAP4; Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor 4; Apoptosis inhibitor survivin |
| Entrez GeneID | 332 |
| WB Predicted band size | Calculated MW: 16 kDa; Observed MW: 16 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human Survivin |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于Survivin抗体的代表性文献摘要信息：
1. **文献名称**: *"Survivin as a prognostic marker in squamous cell carcinoma of the esophagus"*
**作者**: Kato J et al.
**摘要**: 该研究通过免疫组化方法(使用特异性Survivin单克隆抗体)分析食管鳞癌组织中Survivin的表达，发现其高表达与患者生存率降低显著相关，提示Survivin可作为预后标志物。
2. **文献名称**: *"A novel anti-survivin antibody for targeted therapy of solid tumors"*
**作者**: Pennati M et al.
**摘要**: 研究团队开发了一种新型人源化Survivin单克隆抗体(克隆号：AB-123)，通过体外和动物模型验证其抑制肿瘤细胞增殖、促进凋亡的能力，并证实其靶向结合特异性。
3. **文献名称**: *"Validation of survivin antibody specificity in pancreatic cancer diagnosis"*
**作者**: Smith JL et al.
**摘要**: 文章系统评估了多种市售Survivin抗体(包括克隆号D-8和FL-142)在胰腺癌组织中的特异性，通过Western blot和免疫荧光对比，确认FL-142抗体在区分正常/癌变组织中的可靠性。
注：以上文献信息为示例性简化内容，实际引用需根据具体研究检索PubMed等数据库获取完整信息。
Survivin, a member of the inhibitor of apoptosis (IAP) protein family, is a critical regulator of cell survival and proliferation. It functions as a bifunctional protein, suppressing apoptosis by inhibiting caspase activity and ensuring proper cell division by regulating microtubule dynamics during mitosis. Survivin is selectively overexpressed in most human cancers but is nearly undetectable in normal differentiated tissues, making it a promising diagnostic marker and therapeutic target in oncology.
Survivin antibodies are essential tools for detecting and quantifying Survivin expression in research and clinical settings. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence to study Survivin's role in tumor progression, treatment resistance, and prognosis. Specific clones (e.g., DR5-30-H4. 8E2) target distinct Survivin epitopes, with some recognizing both cytoplasmic (cell cycle-associated) and nuclear (mitosis-specific) isoforms. However, interpretation requires caution due to potential cross-reactivity with other IAP family members and splice variants like Survivin-2B or Survivin-ΔEx3.
Recent applications extend to liquid biopsy analysis, where Survivin antibodies help detect circulating tumor cells or exosomal Survivin in cancer patients. As Survivin-targeted therapies (antisense oligonucleotides, small-molecule inhibitors) enter clinical trials, validated antibodies remain crucial for pharmacodynamic studies and companion diagnostics. Researchers must verify antibody specificity using knockout controls and correlate findings with functional assays to ensure biological relevance.
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