首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠肿瘤坏死因子α(TNF-α)抗体酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的TNF-α Ab含量。TNF-α Ab即肿瘤坏死因子-α单克隆抗体，TNF-α在多种炎症疾病中过度表达。TNF-α Ab的研究机制是其能特异性结合TNF-α，阻断它与受体相互作用，减少炎症信号传导。它为类风湿关节炎等炎性疾病治疗带来新方向。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于炎症机制研究、免疫治疗药物体外效果评估、感染性疾病模型分析等科研场景，为研究感染性休克、类风湿性关节炎等TNF-α相关疾病的分子机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：antibody for DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein|TNF superfamily, member 2|TNF, macrophage-derived|TNF, monocyte-derived|cachectin|tumor necrosis factor alpha
缩写：TNF-α Ab
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNF-α antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 87-96 | ||||
| Range % | 82-96 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 88-100 | |||||
回收率：
| The recovery of mouse TNF-α antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-94 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.7132.7522.7332.580 52.2122.3492.2812.128 2.51.5141.6231.5691.416 1.250.8490.8640.8570.704 0.6250.5020.5250.5140.361 0.3120.3130.3160.3150.162 0.1560.2570.2700.2640.111 00.1480.1570.153 | ||||||
货期：3-5 working days
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