| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SMAC; DFNA64; DIABLO; SMAC3 |
| Entrez GeneID | 56616 |
| WB Predicted band size | Calculated MW: 27 kDa; Observed MW: 21 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Smac/Diablo |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于SMAC抗体的3-4篇参考文献及其摘要概括：
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1. **文献名称**：*Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition*
**作者**：Du, C., Fang, M., Li, Y., Li, L., Wang, X.
**摘要**：该研究首次鉴定并描述了SMAC(Second Mitochondria-derived Activator of Caspases)蛋白的功能，证明其在线粒体凋亡途径中通过拮抗凋亡抑制蛋白(IAPs，如XIAP)释放胱天蛋白酶(caspase-9/caspase-3)，从而激活细胞凋亡。实验中利用SMAC抗体验证了其在细胞凋亡时从线粒体释放至胞质的过程。
2. **文献名称**：*Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins*
**作者**：Verhagen, A.M., Ekert, P.G., Pakusch, M., Silke, J., Connolly, L.M., Reid, G.E., Moritz, R.L., Simpson, R.J., Vaux, D.L.
**摘要**：本研究独立发现了DIABLO(即SMAC)，证实其通过直接结合IAPs(如XIAP、c-IAP1)并阻断其抑制凋亡的功能。研究中使用特异性抗体检测DIABLO/SMAC的亚细胞定位及其在凋亡过程中的释放机制。
3. **文献名称**：*Structural basis of IAP recognition by Smac/DIABLO*
**作者**：Wu, G., Chai, J., Suber, T.L., Wu, J.W., Du, C., Wang, X., Shi, Y.
**摘要**：通过X射线晶体学解析SMAC蛋白与XIAP的BIR3结构域复合物结构，揭示了SMAC通过N端四肽(AVPI)与IAPs结合的关键机制。研究中可能利用SMAC抗体进行免疫共沉淀或蛋白质相互作用验证。
4. **文献名称**：*Smac agonists sensitize for Apo2L/TRAIL- or anticancer drug-induced apoptosis and induce regression of malignant glioma in vivo*
**作者**：Fulda, S., Wick, W., Weller, M., Debatin, K.M.
**摘要**：探讨SMAC模拟物(小分子拮抗剂)在增强肿瘤细胞对凋亡诱导剂(如TRAIL)敏感性中的作用，并通过SMAC抗体检测其在胶质瘤模型中的表达及治疗效果。
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这些文献涵盖了SMAC的发现、结构机制及治疗应用，其中抗体的使用多集中于蛋白定位、释放验证及功能研究。如需具体实验细节，建议进一步查阅全文。
**Background of SMAC Antibodies**
SMAC (Second Mitochondrial Activator of Caspases), also known as Diablo, is a mitochondrial protein critical in regulating apoptosis. It is released into the cytosol during apoptosis alongside cytochrome c, triggered by cellular stress or damage. SMAC promotes caspase activation by neutralizing inhibitor of apoptosis proteins (IAPs), such as XIAP, which block caspases and impede cell death. This interaction occurs via SMAC’s N-terminal AVPI motif, binding to IAPs’ BIR domains, thereby derepressing caspases and enabling apoptosis execution.
SMAC antibodies are essential tools for studying apoptosis mechanisms, cancer biology, and therapeutic development. They enable detection of endogenous SMAC levels, localization (e.g., mitochondrial vs. cytosolic fractions), and interactions with IAPs in techniques like Western blotting, immunohistochemistry, or co-immunoprecipitation. In cancer research, SMAC antibodies help evaluate SMAC expression in tumors, where dysregulation may contribute to apoptosis resistance. Additionally, they validate SMAC-mimetic drugs designed to antagonize IAPs and restore apoptosis in cancer cells.
The development of SMAC antibodies has advanced understanding of mitochondrial apoptosis pathways and supported translational studies targeting IAPs for cancer therapy. Their specificity and reliability make them indispensable in both basic research and preclinical drug evaluation.
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