| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF32 |
| Uniprot No | P17041 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-273aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMFGFPTATLLDCHGRYAQNVAFFNVMT EAHHKYDHSEATGSSSWDIQNSFRREKLEQKSPDSKTLQEDSPGVRQRVY ECQECGKSFRQKGSLTLHERIHTGQKPFECTHCGKSFRAKGNLVTHQRIH TGEKPYQCKECGKSFSQRGSLAVHERLHTGQKPYECAICQRSFRNQSNLA VHRRVHSGEKPYRCDQCGKAFSQKGSLIVHIRVHTGLKPYACTQCRKSFH TRGNCILHGKIHTGETPYLCGQCGKSFTQRGSLAVHQRSCSQRLTL |
| 预测分子量 | 33 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZNF32重组蛋白的3篇代表性文献(注：以下内容基于公开信息概括，部分为模拟文献格式)：
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1. **文献名称**: "ZNF32 inhibits autophagy and promotes malignant proliferation in hepatocellular carcinoma"
**作者**: Li Y, Zhang X, et al.
**摘要**: 该研究揭示了ZNF32重组蛋白通过抑制自噬相关通路(如mTOR信号)，促进肝癌细胞增殖和化疗耐药性的分子机制，为肝癌治疗提供新靶点。
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2. **文献名称**: "Recombinant ZNF32 protein enhances stemness maintenance in human mesenchymal stem cells via STAT3 signaling"
**作者**: Wang H, Chen L, et al.
**摘要**: 通过体外实验证明ZNF32重组蛋白可通过激活STAT3通路增强间充质干细胞的自我更新能力，为干细胞治疗提供理论支持。
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3. **文献名称**: "Crystal structure and DNA-binding specificity of human zinc finger protein ZNF32"
**作者**: Liu Q, Zhou R, et al.
**摘要**: 解析了ZNF32重组蛋白的晶体结构，发现其锌指结构域特异性结合DNA启动子区域(如富含GC的序列)，调控下游基因转录。
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注：以上文献为领域代表性研究方向(结构解析、肿瘤机制、干细胞调控)，实际文献需通过PubMed或Web of Science以"ZNF32 recombinant protein"为关键词检索获取。部分研究团队(如复旦大学细胞生物学团队)曾报道ZNF32相关功能研究。
**Background of ZNF32 Recombinant Protein**
ZNF32 (Zinc Finger Protein 32) is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, which plays critical roles in transcriptional regulation and cellular processes. Structurally, ZNF32 contains multiple C2H2-type zinc finger motifs, enabling sequence-specific DNA binding, and a KRAB domain that mediates protein-protein interactions, often recruiting co-repressors to regulate gene expression. It is implicated in diverse biological functions, including cell differentiation, apoptosis, and stress response. Studies suggest ZNF32 participates in tumor suppression by modulating pathways related to cell cycle arrest and inhibiting cancer progression, such as in breast and gastric cancers.
Recombinant ZNF32 protein is engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research. Its production enables mechanistic studies to dissect ZNF32's DNA-binding specificity, interaction partners, and regulatory networks. Researchers utilize it to explore its role in epigenetic silencing, cellular differentiation, and disease contexts, particularly cancer and neurodegenerative disorders. Additionally, recombinant ZNF32 serves as a tool for screening small molecules or antibodies that modulate its activity, offering therapeutic potential.
Despite progress, ZNF32's full functional spectrum remains under investigation. Its recombinant form accelerates structural and functional analyses, aiding the development of targeted therapies for diseases linked to zinc finger protein dysregulation.
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