| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZMAT3 |
| Uniprot No | Q9HA38 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-289aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMILLQHAVLPPPKQPSPSPPMSVATRS TGTLQLPPQKPFGQEASLPLAGEEELSKGGEQDCALEELCKPLYCKLCNV TLNSAQQAQAHYQGKNHGKKLRNYYAANSCPPPARMSNVVEPAATPVVPV PPQMGSFKPGGRVILATENDYCKLCDASFSSPAVAQAHYQGKNHAKRLRL AEAQSNSFSESSELGQRRARKEGNEFKMMPNRRNMYTVQNNSAGPYFNPR SRQRIPRDLAMCVTPSGQFYCSMCNVGAGEEMEFRQHLESKQHKSKVSEQ RYRNEMENLGYV |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZMAT3重组蛋白的3篇参考文献示例(文献信息为虚构，仅供格式参考)：
1. **文献名称**：*ZMAT3 modulates p53-mediated apoptosis through regulation of NF-κB signaling*
**作者**：Chen L, et al.
**摘要**：该研究利用重组ZMAT3蛋白，揭示了其通过抑制NF-κB通路增强p53依赖性凋亡的分子机制，为肿瘤治疗提供了潜在靶点。
2. **文献名称**：*Structural characterization of recombinant ZMAT3 and its RNA-binding activity*
**作者**：Smith JR, et al.
**摘要**：通过体外表达纯化重组ZMAT3蛋白，结合X射线晶体学分析，发现其锌指结构域特异性结合RNA，参与转录后调控。
3. **文献名称**：*ZMAT3 overexpression inhibits tumor growth via metabolic reprogramming*
**作者**：Wang Y, et al.
**摘要**：研究表明，重组ZMAT3蛋白的过表达通过抑制糖酵解关键酶LDHA，诱导癌细胞代谢重编程，从而抑制体内外肿瘤生长。
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如需真实文献，建议在PubMed或Google Scholar中搜索关键词：**ZMAT3 recombinant protein** 或 **ZMAT3 overexpression functional study**。
ZMAT3. also known as Sideroflexin 4 (SFXN4) or P53-inducible apoptosis regulator (PAG608), is a nuclear-encoded protein involved in diverse cellular processes, including apoptosis, senescence, and tumor suppression. It belongs to the zinc finger matrin-type (ZMAT) protein family, characterized by conserved zinc finger domains that mediate nucleic acid binding. ZMAT3 was initially identified as a direct transcriptional target of the tumor suppressor p53. linking it to stress response pathways and genomic stability.
Structurally, ZMAT3 contains two N-terminal C2H2-type zinc finger motifs critical for RNA/DNA interactions and a C-terminal nuclear localization signal. It localizes primarily to the nucleus, where it modulates gene expression by interacting with RNA, DNA, or protein complexes. Studies suggest ZMAT3 regulates alternative splicing, ribosomal RNA processing, and mitochondrial function, though its precise molecular mechanisms remain under investigation.
In cancer biology, ZMAT3 exhibits context-dependent roles. While it often acts as a tumor suppressor by promoting apoptosis and inhibiting cell proliferation in response to p53 activation, some cancers show ZMAT3 overexpression associated with poor prognosis, suggesting potential oncogenic functions in specific settings. Its dual roles may stem from interactions with different partners, such as ribosomal proteins or RNA helicases, influencing cellular stress adaptation.
Recombinant ZMAT3 protein is engineered for in vitro studies to dissect its biochemical properties, structural features, and functional interactions. Produced via bacterial or mammalian expression systems, it enables research on its RNA-binding specificity, role in p53-mediated pathways, and therapeutic potential. Recent work also explores ZMAT3’s involvement in mitochondrial metabolism and ferroptosis, expanding its relevance in neurodegenerative diseases and aging. Ongoing research aims to clarify its multifaceted contributions to cell fate decisions and disease pathogenesis.
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