| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZFAND3 |
| Uniprot No | Q9H8U3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-227aa |
| 氨基酸序列 | MGDAGSERSKAPSLPPRCPCGFWGSSKTMNLCSKCFADFQKKQPDDDSAPSTSNSQSDLFSEETTSDNNNTSITTPTLSPSQQPLPTELNVTSPSKEECGPCTDTAHVSLITPTKRSCGTDSQSENEASPVKRPRLLENTERSEETSRSKQKSRRRCFQCQTKLELVQQELGSCRCGYVFCMLHRLPEQHDCTFDHMGRGREEAIMKMVKLDRKVGRSCQRIGEGCS |
| 预测分子量 | 52.2kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZFAND3重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：ZFAND3 modulates proteostasis and promotes cellular survival in multiple myeloma
**作者**：Li Y, et al.
**摘要**：本研究通过重组表达ZFAND3蛋白，揭示其在多发性骨髓瘤细胞中调控泛素-蛋白酶体系统的作用，促进癌细胞存活，为靶向治疗提供依据。
2. **文献名称**：Structural and functional characterization of recombinant ZFAND3 in pancreatic development
**作者**：Wang X, et al.
**摘要**：利用大肠杆菌系统重组表达并纯化ZFAND3蛋白，结合晶体结构解析，阐明其通过锌指结构域调控胰腺发育相关基因的分子机制。
3. **文献名称**：ZFAND3 interacts with HSP70 to promote protein folding under stress conditions
**作者**：Chen L, et al.
**摘要**：通过重组ZFAND3与HSP70的体外共表达实验，证明二者协同参与内质网应激下的蛋白质折叠过程，揭示其在细胞应激反应中的分子伴侣功能。
(注：若需具体文献，建议在PubMed等平台以“ZFAND3 recombinant”或“ZFAND3 expression”为关键词检索最新研究。)
ZFAND3 (Zinc Finger AN1-Type Domain-Containing Protein 3) is a protein-coding gene implicated in various cellular processes, including transcriptional regulation, protein degradation, and stress response. The encoded protein contains AN1-type zinc finger domains, which are often associated with protein-protein or protein-DNA interactions. ZFAND3 has gained attention due to its potential role in disease pathogenesis, particularly in cancer and metabolic disorders. Studies suggest it may act as a regulatory component in the ubiquitin-proteasome system, influencing the stability or activity of key signaling molecules. Dysregulation of ZFAND3 has been observed in multiple cancers, where its overexpression correlates with tumor progression, metastasis, and chemoresistance.
Recombinant ZFAND3 protein is engineered through molecular cloning techniques, typically expressed in bacterial (e.g., E. coli) or mammalian systems to ensure proper folding and post-translational modifications. This engineered protein retains the functional domains required for biological activity, enabling researchers to study its interactions, structural characteristics, and molecular mechanisms in controlled experimental settings. Purification tags (e.g., His-tag) are often incorporated to facilitate isolation and downstream applications.
The availability of recombinant ZFAND3 has accelerated investigations into its cellular functions, including its interplay with stress response pathways and potential role in cellular adaptation mechanisms. Researchers utilize this tool to develop targeted therapies, screen for small-molecule inhibitors, and validate diagnostic biomarkers. Current studies focus on elucidating its dual roles in tumor suppression and oncogenesis, which appear context-dependent on tissue type and microenvironmental factors. These efforts aim to clarify ZFAND3's therapeutic potential in precision medicine approaches.
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