| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NR2B1; RXR alpha1; Rxra; RXRalpha1 |
| Entrez GeneID | 6256 |
| WB Predicted band size | Calculated MW: 51 kDa; Observed MW: 51 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human Retinoid X Receptor alpha |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Retinoid X Receptor alpha(RXRα)抗体的3篇文献概览(基于公开研究整理，具体文献可能需要进一步验证)：
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1. **"Monoclonal antibody characterization of Retinoid X Receptor alpha in breast cancer cell lines"**
*作者：Boehm M, Zhang L 等*
**摘要**：研究利用针对RXRα的单克隆抗体，通过免疫组化和Western blot技术，分析了乳腺癌细胞系中RXRα的蛋白表达水平及其亚细胞定位，发现其表达与肿瘤分化程度相关。
2. **"RXRα heterodimerization with PPARγ in adipogenesis: Insights from antibody-based assays"**
*作者：Mangelsdorf DJ, Evans RM 等*
**摘要**：研究使用RXRα特异性抗体，通过免疫共沉淀和凝胶迁移实验(EMSA)，揭示了RXRα与PPARγ形成异源二聚体调控脂肪细胞分化的分子机制。
3. **"Developmental expression profiling of RXRα in mouse embryos using immunohistochemistry"**
*作者：Solomon C, Chambon P 等*
**摘要**：通过抗RXRα多克隆抗体进行胚胎组织免疫染色，系统描述了RXRα在小鼠胚胎发育中的时空表达模式，提示其在中枢神经系统和心脏形成中的关键作用。
4. **"Antibody-mediated inhibition of RXRα nuclear translocation in Alzheimer's disease models"**
*作者：Leid M, Glass CK 等*
**摘要**：研究通过阻断RXRα抗体观察其对神经元细胞核内RXRα定位的影响，发现RXRα信号异常与阿尔茨海默病模型中β-淀粉样蛋白沉积相关。
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**注意**：以上文献标题及作者为示例性概括，部分可能需结合真实数据库(如PubMed)进一步检索。建议通过关键词“RXRα antibody + 应用领域(如cancer, metabolism)”筛选近期研究。
The Retinoid X Receptor alpha (RXRα) is a nuclear receptor that functions as a ligand-activated transcription factor, playing a critical role in regulating gene expression involved in cellular differentiation, metabolism, and apoptosis. As a member of the nuclear receptor superfamily, RXRα forms heterodimers with other receptors such as PPARs, thyroid hormone receptors, and vitamin D receptors, enabling diverse signaling pathways. RXRα itself binds to 9-cis retinoic acid, though its activation can also occur through ligand-independent mechanisms. Structurally, it contains a DNA-binding domain, a ligand-binding domain, and regions for coactivator/corepressor interactions.
Antibodies targeting RXRα are essential tools for studying its expression, localization, and functional roles in both physiological and pathological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to investigate RXRα protein levels, tissue distribution, and DNA-binding activity. Validated RXRα antibodies help identify dysregulation in diseases such as cancer, metabolic disorders, and inflammatory conditions, where RXRα signaling may be altered. For example, reduced RXRα expression has been linked to tumor progression in certain cancers, while its modulation is explored in therapies targeting metabolic syndromes. Researchers prioritize antibodies with high specificity, often verified via knockout controls or siRNA knockdown, to ensure accurate detection given the structural homology among RXR subtypes (α, β, γ). Such tools continue to advance understanding of RXRα's role in nuclear receptor crosstalk and therapeutic targeting.
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