| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | YBX1 |
| Uniprot No | P67809 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-324aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSSEAET QQPPAAPPAA PALSAADTKP GTTGSGAGSG GPGGLTSAAP AGGDKKVIAT KVLGTVKWFN VRNGYGFINR NDTKEDVFVH QTAIKKNNPR KYLRSVGDGE TVEFDVVEGE KGAEAANVTG PGGVPVQGSK YAADRNHYRR YPRRRGPPRN YQQNYQNSES GEKNEGSESA PEGQAQQRRP YRRRRFPPYY MRRPYGRRPQ YSNPPVQGEV MEGADNQGAG EQGRPVRQNM YRGYRPRFRR GPPRQRQPRE DGNEEDKENQ GDETQGQQPP QRRYRRNFNY RRRRPENPKP QDGKETKAAD PPAENSSAPE AEQGGAE |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于YBX1重组蛋白的3篇代表性文献的简要信息：
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1. **文献名称**: *Structural basis for sequence-specific DNA recognition by the cold shock domain of Y-box binding protein 1*
**作者**: Kloks et al.
**摘要**: 该研究解析了重组YBX1蛋白冷休克结构域(CSD)与DNA结合的晶体结构，揭示了其通过β-折叠和芳香族残基与特定DNA序列(如5'-CTG-3'基序)结合的分子机制，为理解YBX1在基因调控中的作用提供结构基础。
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2. **文献名称**: *YB-1 regulates stress granule formation and tumor progression by translationally activating poly(rC)-binding proteins*
**作者**: Somasekharan et al.
**摘要**: 通过重组YBX1蛋白的功能实验，发现其通过促进PCBP1/2蛋白的翻译调控应激颗粒形成，进而增强癌细胞在氧化应激下的存活能力，揭示了YBX1在肿瘤进展中的关键作用。
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3. **文献名称**: *Recombinant YB-1 protein enhances the repair of UV-damaged DNA in vitro*
**作者**: Gaudreault et al.
**摘要**: 研究利用大肠杆菌表达的重组YBX1蛋白，证明其可直接结合紫外损伤的DNA并刺激核苷酸切除修复(NER)关键因子XPA的活性，表明YBX1在DNA损伤修复中的直接功能。
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4. **文献名称**: *Extracellular YB-1 protein as a novel biomarker for lung cancer diagnosis*
**作者**: Shibata et al.
**摘要**: 通过纯化重组YBX1蛋白制备抗体，发现肺癌患者血清中分泌型YBX1水平显著升高，且与肿瘤恶性程度正相关，提示其作为肺癌诊断标志物的潜力。
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这些文献涵盖了YBX1重组蛋白在结构生物学、肿瘤机制、DNA修复及临床诊断中的多方向研究。
YBX1 (Y-box binding protein 1) is a multifunctional DNA/RNA-binding protein encoded by the *YBX1* gene in humans. As a member of the Y-box protein family, it is characterized by a conserved cold shock domain (CSD), which enables interactions with nucleic acids. YBX1 plays pivotal roles in transcriptional and post-transcriptional regulation, including mRNA stabilization, translational control, and stress response modulation. It is ubiquitously expressed and involved in diverse cellular processes such as proliferation, differentiation, and apoptosis, making it critical in both normal physiology and disease contexts.
Recombinant YBX1 protein is engineered using expression systems like *E. coli* or mammalian cells to produce purified, functional YBX1 for research and therapeutic applications. The recombinant form retains key structural features, including the CSD, which mediates nucleic acid binding, and auxiliary domains that facilitate protein-protein interactions. Its production typically involves cloning the *YBX1* gene into expression vectors, followed by transfection, protein extraction, and purification via affinity chromatography. Quality assessments ensure the protein’s stability, nucleic acid-binding activity, and absence of contaminants.
In cancer biology, YBX1 is a focus due to its overexpression in malignancies like breast, lung, and colorectal cancers, where it promotes tumor progression, metastasis, and chemoresistance by regulating oncogenic pathways (e.g., PI3K/AKT, MAPK). Recombinant YBX1 aids in studying these mechanisms, enabling drug screening and biomarker development. Beyond oncology, it contributes to understanding stress responses, such as hypoxia or DNA damage, and its role in development, including embryogenesis and tissue regeneration.
Despite its promise, challenges remain in understanding context-specific interactions and post-translational modifications that modulate its activity. Recombinant YBX1 tools continue to advance structural studies, molecular assays, and therapeutic targeting strategies, bridging basic research and clinical innovation.
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