| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | BCAR1 |
| Uniprot No | P56945 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-870aa |
| 氨基酸序列 | MNHLNVLAKALYDNVAESPDELSFRKGDIMTVLEQDTQGLDGWWLCSLHG RQGIVPGNRLKILVGMYDKKPAGPGSGPPATPAQPQPGLHAPAPPASQYT PMLPNTYQPQPDSVYLVPTPSKAQQGLYQVPGPSPQFQSPPAKQTSTFSK QTPHHPFPNPATDLYQVPPGPGGPAQDIYQVPPSAGMGHDIYQVPPSMDT RSWEGTKPPAKVVVPTRVGQGYVYEAAQPEQDEYDIPRHLLAPGPQDIYD VPPVRGLLPSQYGQEVYDTPPMAVKGPNGRDPLLEVYDVPPSVEKGLPPS NHHAVYDVPPSVSKDVPDGPLLREETYDVPPAFAKAKPFDPARTPLVLAA PPPDSPPAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVA DGGVVDSGVYAVPPPAEREAPAEGKRLSASSTGSTRSSQSASSLEVAGPG REPLELEVAVEALARLQQGVSATVAHLLDLAGSAGATGSWRSPSEPQEPL VQDLQAAVAAVQSAVHELLEFARSAVGNAAHTSDRALHAKLSRQLQKMED VHQTLVAHGQALDAGRGGSGATLEDLDRLVACSRAVPEDAKQLASFLHGN ASLLFRRTKATAPGPEGGGTLHPNPTDKTSSIQSRPLPSPPKFTSQDSPD GQYENSEGGWMEDYDYVHLQGKEEFEKTQKELLEKGSITRQGKSQLELQQ LKQFERLEQEVSRPIDHDLANWTPAQPLAPGRTGGLGPSDRQLLLFYLEQ CEANLTTLTNAVDAFFTAVATNQPPKIFVAHSKFVILSAHKLVFIGDTLS RQAKAADVRSQVTHYSNLLCDLLRGIVATTKAAALQYPSPSAAQDMVERV KELGHSTQQFRRVLGQLAAA |
| 预测分子量 | 120 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BCAR1重组蛋白的虚构参考文献示例(仅供格式参考，非真实文献)：
1. **《Recombinant BCAR1 Protein Enhances Estrogen-Independent Breast Cancer Cell Proliferation》**
- 作者：Lee, J. et al.
- 摘要：研究通过大肠杆菌系统成功表达并纯化BCAR1重组蛋白，证实其通过激活Src/FAK信号通路促进乳腺癌细胞在无雌激素环境中的增殖，为耐药机制提供新见解。
2. **《Structural Characterization of BCAR1 Recombinant Protein Using Cryo-EM》**
- 作者：Chen, X. & Wang, Y.
- 摘要：首次报道BCAR1重组蛋白的冷冻电镜结构，揭示其Cas家族典型结构域(SH3结合域和螺旋结构域)的构象变化，解析其与CrkII相互作用的分子基础。
3. **《BCAR1 Recombinant Protein as a Biomarker for Tamoxifen Resistance》**
- 作者：Rodriguez, M. et al.
- 摘要：通过体外实验证明，高表达的重组BCAR1蛋白与乳腺癌细胞对他莫昔芬的耐药性呈正相关，提出其可作为临床耐药性预测的潜在标志物。
4. **《Optimization of BCAR1 Recombinant Protein Expression in Mammalian Cells》**
- 作者：Tanaka, H. et al.
- 摘要：对比不同哺乳动物表达系统(HEK293 vs. CHO)中BCAR1重组蛋白的产量与活性，发现HEK293系统更适用于功能性磷酸化BCAR1的大规模制备。
注：以上内容为模拟示例，实际文献需通过学术数据库检索获取。
BCAR1 (Breast Cancer Anti-estrogen Resistance 1), also known as p130Cas, is a scaffold protein implicated in cell signaling pathways regulating adhesion, migration, and survival. Initially identified for its role in conferring resistance to anti-estrogen therapies in breast cancer, BCAR1 has since been recognized as a critical adaptor molecule in diverse physiological and pathological processes. Structurally, it contains multiple protein interaction domains, including an N-terminal SH3 domain, a central substrate domain with tyrosine phosphorylation sites, a serine-rich region, and a C-terminal CAS family homology domain. These domains enable BCAR1 to interact with signaling molecules such as Src family kinases, focal adhesion kinase (FAK), and integrins, facilitating cross-talk between extracellular matrix signals and intracellular signaling cascades.
BCAR1 is a key mediator of integrin-mediated signaling, influencing cytoskeletal reorganization, cell proliferation, and epithelial-mesenchymal transition (EMT). Its phosphorylation status dynamically regulates downstream effectors like Crk, PI3K/Akt, and MAPK pathways, linking mechanical cues to transcriptional responses. Dysregulation of BCAR1 is associated with cancer progression, metastasis, and therapy resistance. Overexpression or hyperactivation of BCAR1 has been observed in breast, ovarian, and lung cancers, correlating with poor prognosis.
Recombinant BCAR1 protein is engineered for in vitro studies to dissect its molecular interactions, phosphorylation dynamics, and structural-functional relationships. Produced via bacterial or mammalian expression systems, it serves as a tool for investigating signaling mechanisms, screening therapeutic inhibitors, and developing diagnostic biomarkers. Research on recombinant BCAR1 continues to advance understanding of its role in cancer biology and its potential as a therapeutic target.
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