首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人氧化低密度脂蛋白抗体(OLAb)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的ox-LDL Ab含量。ox-LDL Ab即氧化低密度脂蛋白抗体。其背景在于氧化低密度脂蛋白与动脉粥样硬化等疾病密切相关，机体产生相应抗体。研究机制上，主要探索其与疾病进程的关联，如在斑块形成、炎症反应中的作用，以明确其作为诊断标志物或治疗靶点的价值。试剂盒检测范围为47 μU/mL-3000 μU/mL，该产品适用于心血管疾病模型构建、代谢异常相关研究或炎症反应机制分析等科研场景，为评估氧化脂蛋白在疾病发展中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：ox-LDL Ab
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：47 μU/mL-3000 μU/mL
灵敏度：11.75 μU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human OLAb in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 90 | ||||
| Range % | 87-98 | |||||
| 1:2000 | Average % | 93 | ||||
| Range % | 91-99 | |||||
| 1:4000 | Average % | 95 | ||||
| Range % | 90-102 | |||||
| 1:8000 | Average % | 92 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human OLAb spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 86-98 | ||||
| EDTA plasma (n=4) | 96 | 91-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μU/mlOD1OD2AverageCorrected 30002.5322.5172.5252.401 15002.2332.2592.2462.122 7501.6971.6441.6711.547 3751.0511.0491.0500.926 187.50.6510.6150.6330.509 940.3850.3920.3890.265 470.2480.2510.2500.126 00.1220.1250.124 | ||||||
货期：3-5 working days
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