| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | XPNPEP1 |
| Uniprot No | Q9NQW7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-623aa |
| 氨基酸序列 | PPKVTSELLRQLRQAMRNSEYVTEPIQAYIIPSGDAHQSEYIAPCDCRRA FVSGFDGSAGTAIITEEHAAMWTDGRYFLQAAKQMDSNWTLMKMGLKDTP TQEDWLVSVLPEGSRVGVDPLIIPTDYWKKMAKVLRSAGHHLIPVKENLV DKIWTDRPERPCKPLLTLGLDYTGISWKDKVADLRLKMAERNVMWFVVTA LDEIAWLFNLRGSDVEHNPVFFSYAIIGLETIMLFIDGDRIDAPSVKEHL LLDLGLEAEYRIQVHPYKSILSELKALCADLSPREKVWVSDKASYAVSET IPKDHRCCMPYTPICIAKAVKNSAESEGMRRAHIKDAVALCELFNWLEKE VPKGGVTEISAADKAEEFRRQQADFVDLSFPTISSTGPNGAIIHYAPVPE TNRTLSLDEVYLIDSGAQYKDGTTDVTRTMHFGTPTAYEKECFTYVLKGH IAVSAAVFPTGTKGHLLDSFARSALWDSGLDYLHGTGHGVGSFLNVHEGP CGISYKTFSDEPLEAGMIVTDEPGYYEDGAFGIRIENVVLVVPVKTKYNF NNRGSLTFEPLTLVPIQTKMIDVDSLTDKECDWLNNYHLTCRDVIGKELQ KQGRQEALEWLIRETQPISKQH |
| 预测分子量 | 70 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于XPNPEP1重组蛋白的参考文献摘要示例(注：文献为假设性示例，实际引用需核实原始文献)：
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1. **文献名称**：*"Expression and enzymatic characterization of recombinant human XPNPEP1 in Escherichia coli"*
**作者**：Smith J et al.
**摘要**：本研究成功在大肠杆菌中表达了重组人源XPNPEP1蛋白，并优化了纯化条件。通过体外酶活实验证实其特异性水解X-Pro键的氨肽酶活性，为进一步研究其在血管紧张素代谢中的作用提供了工具。
2. **文献名称**：*"Structural insights into XPNPEP1 substrate recognition using recombinant protein crystallography"*
**作者**：Lee S et al.
**摘要**：利用重组表达的XPNPEP1蛋白进行晶体结构解析，揭示了其与底物类似物的结合模式，阐明了该酶对脯氨酸残基的选择性机制，为设计靶向抑制剂提供了结构基础。
3. **文献名称**：*"XPNPEP1 deficiency alters bradykinin metabolism: Functional validation with recombinant protein assays"*
**作者**：Garcia R et al.
**摘要**：通过重组XPNPEP1蛋白的体外功能实验，证明其参与缓激肽降解通路，并发现基因突变导致酶活性丧失可能与遗传性血管性水肿相关，提示其潜在治疗靶点价值。
4. **文献名称**：*"High-throughput screening of XPNPEP1 inhibitors using a fluorescent recombinant substrate system"*
**作者**：Wang Y et al.
**摘要**：开发基于重组XPNPEP1蛋白的荧光底物检测体系，筛选出多种小分子抑制剂，为治疗XPNPEP1异常相关的肾脏疾病提供了先导化合物。
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以上内容综合了重组蛋白表达、结构功能研究、疾病机制探索及药物开发等方向，符合典型研究框架。实际应用中需根据具体研究领域补充真实文献。
XPNPEP1 (X-prolyl aminopeptidase 1) is a metalloprotease encoded by the XPNPEP1 gene in humans, primarily involved in the metabolism of bioactive peptides. It belongs to the M24B subfamily of aminopeptidases and plays a critical role in cleaving N-terminal Xaa-Pro bonds in peptides such as bradykinin, collagen fragments, and immunomodulatory peptides. This enzyme exists in two isoforms: a mitochondrial form and a cytosolic form, generated through alternative splicing. Its activity is linked to the regulation of peptide-mediated physiological processes, including blood pressure modulation, immune response, and extracellular matrix remodeling.
Recombinant XPNPEP1 protein is engineered using biotechnological platforms (e.g., bacterial, insect, or mammalian expression systems) to produce purified, functional enzyme for research and therapeutic applications. Its recombinant form enables detailed study of substrate specificity, catalytic mechanisms, and structural characteristics. Researchers utilize it to investigate XPNPEP1's role in diseases such as hypertension, inflammatory disorders, and kidney dysfunction, where dysregulation of peptide metabolism is implicated. For instance, altered bradykinin degradation due to XPNPEP1 variants has been associated with ACE inhibitor-induced angioedema.
The production of recombinant XPNPEP1 also supports drug discovery efforts, particularly in designing inhibitors or modulators targeting peptide-mediated pathways. Structural studies using recombinant protein have revealed its dependence on metal ions (e.g., manganese) for enzymatic activity and provided insights into its substrate-binding pocket. Additionally, recombinant XPNPEP1 serves as a tool to validate genetic mutations affecting enzyme function in clinical diagnostics. Its applications extend to biomarker studies, given its involvement in metabolizing peptides with pathological significance.
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