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产品描述：人水通道蛋白4抗体(AQP-4 Ab)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的AQP-4 Ab 含量。AQP - 4 Ab是一种靶点。AQP - 4即水通道蛋白4，其抗体（Ab）在神经系统疾病尤其视神经脊髓炎谱系疾病中意义重大。研究机制上，它可结合AQP - 4，介导补体激活、免疫细胞浸润等免疫病理过程，损伤神经组织。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于科研场景中疾病模型构建、抗体表达水平研究、药物干预效果评估等实验需求；为探索 AQP - 4 抗体在神经免疫疾病中的分子机制、筛选潜在治疗靶点等基础研究提供可靠工具，检测数据可服务于细胞实验、动物模型研究及生物标志物分析等方向。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：AQP-4 Ab
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AQP-4 antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 82-95 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 84-105 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 82-97 | |||||
回收率：
| The recovery of human AQP-4 antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 82-197 | ||||
| EDTA plasma (n=4) | 95 | 84-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 1002.6152.5752.5952.456 501.9882.1032.0461.907 251.2991.3121.3061.167 12.50.6720.6750.6740.535 6.250.4070.3920.4000.261 3.120.2940.2780.2860.147 1.560.2250.2140.2200.081 00.1420.1350.139 | ||||||
货期：3-5 working days
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