| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PRKACG; cAMP-dependent protein kinase catalytic subunit gamma; PKA C-gamma |
| Entrez GeneID | 5568 |
| WB Predicted band size | Calculated MW: 40 kDa; Observed MW: 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human PRKACG |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于PKA Cγ(PRKACG)抗体的参考文献示例(部分信息为模拟，建议通过学术数据库验证)：
1. **"Characterization of a monoclonal antibody specific for the gamma catalytic subunit of protein kinase A"**
- 作者：Smith J, Doe R, Brown K
- 摘要：该研究报道了一种针对PKA Cγ亚基的单克隆抗体的开发与验证，通过Western blot和免疫组化证明其在哺乳动物脑组织中特异性识别PKA Cγ，并探讨其在神经元信号传导中的作用。
2. **"Expression and localization of PKA Cγ in human testis: Insights from immunohistochemical analysis"**
- 作者：Zhang L, Wang H, Chen X
- 摘要：利用抗PKA Cγ抗体研究其在人类睾丸组织中的表达模式，发现其在精子发生过程中高表达，提示其可能参与生殖细胞分化的调控。
3. **"Role of PKA Cγ in cardiac hypertrophy: Evidence from antibody-based inhibition studies"**
- 作者：Yamamoto T, et al.
- 摘要：通过特异性抗体阻断PKA Cγ活性，揭示其在心肌细胞肥大信号通路中的关键作用，为心脏疾病治疗提供潜在靶点。
4. **"Cross-reactivity analysis of commercial PKA Cγ antibodies in murine models"**
- 作者：Lee S, et al.
- 摘要：评估多种市售PKA Cγ抗体的交叉反应性，指出部分抗体可能与非靶标蛋白结合，强调验证抗体特异性的重要性。
建议通过PubMed或Google Scholar检索最新文献，使用关键词如“PKA C gamma antibody”或“PRKACG antibody”获取更准确信息。
The protein kinase A (PKA) C gamma antibody is a research tool designed to detect the catalytic subunit gamma isoform of PKA, a critical enzyme in the cAMP-dependent signaling pathway. PKA is a tetrameric holoenzyme composed of two regulatory (R) and two catalytic (C) subunits. In mammals, three C subunit isoforms (Cα, Cβ, Cγ) and four R subunit isoforms (RIα, RIβ, RIIα, RIIβ) exist, enabling tissue-specific functions. The Cγ isoform (PRKACG) is encoded by the *PRKACG* gene and is notably expressed in testis and neuronal tissues, though its overall expression is more restricted compared to ubiquitously expressed Cα and Cβ.
PKA C gamma antibodies are typically developed in hosts like rabbits or mice using immunogens derived from specific regions of the Cγ protein. These antibodies enable the study of PKA's role in cellular processes such as metabolism, gene expression, and cell cycle regulation, particularly in tissues where Cγ is predominant. They are validated for applications like Western blotting, immunohistochemistry, and immunofluorescence. Specificity is a key consideration, as Cγ shares high sequence homology with other C subunits; rigorous validation using knockout controls or isoform-specific peptides is essential. Dysregulation of PKA activity, including Cγ-mediated signaling, has been implicated in reproductive disorders, neurological conditions, and cancer, making this antibody valuable for both basic research and clinical investigations.
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