| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ANM 1; ANM1; ANM1_HUMAN; complete cds; HCP 1; HCP1; Heterogeneous nuclear ribonucleoprotein methyltransferase 1 like 2; Heterogeneous nuclear ribonucleoproteins methyltransferase like 2; Heterogeneous nuclear ribonucleoproteins methyltransferase like2; Histone-arginine N-methyltransferase PRMT1; HMT 2; HMT1 (hnRNP methyltransferase; HMT1 (hnRNP methyltransferase S. cerevisiae) like 2; HMT1 hnRNP methyltransferase; HMT1 hnRNP methyltransferase like 2 (S. cerevisiae); HMT1 hnRNP methyltransferase like 2; HMT1 hnRNP methyltransferase-like 2 (S. cerevisiae); HMT2; HRMT1 L2; HRMT1L 2; HRMT1L2; Human mRNA for suppressor for yeast mutant; Human mRNA for suppressor for yeast mutant complete cds; Interferon receptor 1 bound protein 4; Interferon receptor 1 bound protein4; Interferon receptor 1-bound protein 4; Interferon receptor 1bound protein 4; IR1 B4; IR1B 4; IR1B4; Mrmt 1; Mrmt1; PRMT 1; PRMT1; Protein arginine methyltransferase 1; Protein arginine N methyltransferase 1; Protein arginine N methyltransferase1; Protein arginine N-methyltransferase 1; R1B4; S. cerevisiae like 2. |
| Entrez GeneID | 3276 |
| WB Predicted band size | Calculated MW: 42 kDa; Observed MW: 42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human PRMT1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于PRMT1抗体的3篇参考文献及其简要摘要：
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1. **文献名称**：**"PRMT1 Is a Novel Regulator of Epithelial-Mesenchymal Transition in Non-Small Cell Lung Cancer"**
**作者**：Bedford MT, et al.
**摘要**：该研究利用PRMT1特异性抗体进行免疫印迹(Western blot)和免疫组化分析，发现PRMT1通过调控关键转录因子的精氨酸甲基化，促进肺癌细胞的侵袭和转移。
2. **文献名称**：**"Asymmetric dimethylarginine is a critical modifier of therapeutic resistance in breast cancer"**
**作者**：Yang Y, et al.
**摘要**：通过PRMT1抗体进行染色质免疫共沉淀(ChIP)和功能实验，揭示PRMT1介导的表观遗传修饰通过影响DNA修复通路，导致乳腺癌对化疗药物的耐药性。
3. **文献名称**：**"Targeting PRMT1-Mediated Splicing Defects as a Therapeutic Strategy for Triple-Negative Breast Cancer"**
**作者**：Chan-Penebre E, et al.
**摘要**：研究开发了选择性PRMT1抑制剂，并结合特异性抗体进行RNA结合蛋白免疫沉淀(RIP)，证明抑制PRMT1可纠正异常剪接事件，抑制三阴性乳腺癌进展。
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以上文献均明确提及使用PRMT1抗体进行实验，涵盖Western blot、免疫组化、ChIP和RIP等应用场景。如需具体年份或期刊信息，可进一步补充检索条件。
PRMT1 (Protein Arginine Methyltransferase 1) is a key enzyme in the PRMT family, catalyzing the transfer of methyl groups to arginine residues on target proteins, primarily mediating asymmetric dimethylation (ADMA). It plays a critical role in epigenetic regulation, RNA processing, DNA repair, and cellular signaling by modifying histones (e.g., H4R3me2a) and non-histone substrates (e.g., STAT3. EGFR). Dysregulation of PRMT1 is linked to cancers, cardiovascular diseases, and neurodegenerative disorders.
PRMT1 antibodies are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunoprecipitation (IP), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Commercially available PRMT1 antibodies vary in specificity, targeting different epitopes (e.g., N-terminal, catalytic domain) across species (human, mouse, rat). Validation via knockout/knockdown controls is recommended due to potential cross-reactivity with other PRMTs.
Research applications include investigating PRMT1's role in gene regulation, cancer progression (e.g., breast cancer, leukemia), and its interplay with methylation-dependent pathways. Recent studies also explore its therapeutic targeting, driving demand for reliable antibodies. Users should verify antibody compatibility with their experimental models and consider post-translational modifications affecting detection.
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