| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | XAB1 |
| Uniprot No | Q9HCN4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-374aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMAASAA AAELQASGGP RHPVCLLVLG MAGSGKTTFV QRLTGHLHAQ GTPPYVINLD PAVHEVPFPA NIDIRDTVKY KEVMKQYGLG PNGGIVTSLN LFATRFDQVM KFIEKAQNMS KYVLIDTPGQ IEVFTWSASG TIITEALASS FPTVVIYVMD TSRSTNPVTF MSNMLYACSI LYKTKLPFIV VMNKTDIIDH SFAVEWMQDF EAFQDALNQE TTYVSNLTRS MSLVLDEFYS SLRVVGVSAV LGTGLDELFV QVTSAAEEYE REYRPEYERL KKSLANAESQ QQREQLERLR KDMGSVALDA GTAKDSLSPV LHPSDLILTR GTLDEEDEEA DSDTDDIDHR VTEESHEEPA FQNFMQESMA QYWKRNNK |
| 预测分子量 | 44 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于XAB1重组蛋白相关研究的虚构参考文献示例(注：部分内容可能根据真实研究推断，建议通过学术数据库核实)：
1. **《XAB1 Recombinant Protein Production and Interaction with XPA in DNA Repair》**
Kuraoka, I., et al.
*Journal of Biological Chemistry (2000)*
摘要：研究通过大肠杆菌系统重组表达XAB1蛋白，证实其与XPA(Xeroderma Pigmentosum group A)蛋白的相互作用，并证明其在核苷酸切除修复(NER)中的功能，尤其是在转录偶联修复途径中的调控作用。
2. **《Structural Characterization of Recombinant XAB1 and Its Role in RNA Splicing》**
Nishida, C., et al.
*Nucleic Acids Research (2005)*
摘要：利用X射线晶体学解析重组XAB1蛋白的三维结构，发现其具有RNA识别基序(RRM)，并通过体外实验证明XAB1参与RNA剪接过程，可能作为DNA修复与RNA代谢的交叉调控因子。
3. **《Recombinant XAB1 Expression in Mammalian Cells and Functional Analysis》**
Buchko, G.W., et al.
*Protein Expression and Purification (2012)*
摘要：优化哺乳动物细胞中XAB1重组蛋白的高效表达与纯化方法，验证其与RNA聚合酶II的相互作用，并探讨其在DNA损伤应激条件下的细胞定位变化及功能调控机制。
4. **《XAB1 Knockout and Rescue Experiments Using Recombinant Protein》**
Zhou, Y., et al.
*Cell Reports (2018)*
摘要：通过CRISPR敲除XAB1基因导致细胞对紫外线敏感性增加，而外源重组XAB1蛋白可恢复修复能力。研究揭示了XAB1在维持基因组稳定性中的双重作用(DNA修复与RNA加工)。
**注意**：以上文献为示例性质，实际研究请以PubMed、Web of Science等数据库检索结果为准。真实研究中XAB1可能涉及不同命名(如XAB2为已知基因，XAB1需确认)。
XAB1 recombinant protein, derived from the Xenopus Adenovirus Binding Protein 1 (XAB1), is a genetically engineered protein initially identified for its role in DNA repair and viral-host interactions. Originally isolated in *Xenopus laevis*, XAB1 was found to bind adenovirus DNA and participate in nucleotide excision repair (NER) pathways, a critical cellular mechanism for correcting UV-induced DNA lesions and maintaining genomic stability. Its human homolog, XAB1 (also called HCNP or HMGB1-Caring Nucleotide-binding Protein), shares functional similarities, including interactions with high-mobility group box 1 (HMGB1) proteins, which regulate inflammation, DNA repair, and apoptosis.
Recombinant XAB1 is produced via expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and activity for research and therapeutic applications. Studies highlight its dual role: as a DNA repair facilitator and a modulator of viral replication. For instance, XAB1 binds to HIV-1 integrase, influencing viral integration efficiency, suggesting potential as a target for antiviral strategies. Its involvement in NER also links it to cancer research, particularly in understanding chemotherapy resistance tied to enhanced DNA repair in tumors.
Current research focuses on leveraging XAB1 recombinant protein to dissect DNA-protein interactions, screen antiviral compounds, and explore synergistic therapies combining DNA-damaging agents with XAB1 inhibitors. Challenges include optimizing its stability in vitro and clarifying tissue-specific regulatory mechanisms. As a tool, recombinant XAB1 bridges molecular virology, oncology, and gene editing, offering insights into host-pathogen dynamics and precision medicine development.
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