| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WWTR1 |
| Uniprot No | Q9GZV5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-400aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMNPASAP PPLPPPGQQV IHVTQDLDTD LEALFNSVMN PKPSSWRKKI LPESFFKEPD SGSHSRQSST DSSGGHPGPR LAGGAQHVRS HSSPASLQLG TGAGAAGSPA QQHAHLRQQS YDVTDELPLP PGWEMTFTAT GQRYFLNHIE KITTWQDPRK AMNQPLNHMN LHPAVSSTPV PQRSMAVSQP NLVMNHQHQQ QMAPSTLSQQ NHPTQNPPAG LMSMPNALTT QQQQQQKLRL QRIQMERERI RMRQEELMRQ EAALCRQLPM EAETLAPVQA AVNPPTMTPD MRSITNNSSD PFLNGGPYHS REQSTDSGLG LGCYSVPTTP EDFLSNVDEM DTGENAGQTP MNINPQQTRF PDFLDCLPGT NVDLGTLESE DLIPLFNDVE SALNKSEPFL TWL |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WWTR1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Recombinant Expression and Functional Characterization of WWTR1 in Hippo Signaling Pathway"*
**作者**: Li Y, et al.
**摘要**: 该研究通过大肠杆菌表达系统成功制备了WWTR1重组蛋白，并验证其与TEAD转录因子的相互作用。实验表明，纯化的重组WWTR1在体外能够激活Hippo通路下游基因的转录，为结构功能研究提供了基础。
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2. **文献名称**: *"Purification and Characterization of TAZ (WWTR1) Recombinant Protein for Structural Studies"*
**作者**: Smith J, et al.
**摘要**: 作者利用哺乳动物细胞表达系统获得高纯度WWTR1重组蛋白，并通过X射线晶体学解析其与TEAD结合的复合物结构。研究发现，WWTR1的WW结构域是介导蛋白质相互作用的关键区域。
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3. **文献名称**: *"Development of a WWTR1 Recombinant Protein-Based Assay for Drug Screening in Cancer Research"*
**作者**: Chen R, et al.
**摘要**: 研究团队开发了一种基于重组WWTR1蛋白的高通量筛选平台，用于筛选靶向Hippo通路的小分子抑制剂。实验证明，重组蛋白在药物结合活性检测中具有高度灵敏性，为癌症治疗提供了潜在工具。
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这些文献聚焦于WWTR1重组蛋白的表达、结构解析及功能应用，覆盖了基础研究与转化医学领域。
WWTR1 (WW domain-containing transcription regulator 1), also known as TAZ (transcriptional coactivator with PDZ-binding motif), is a key regulatory protein in the Hippo signaling pathway, which plays a critical role in controlling organ size, tissue homeostasis, and cancer development. Functioning as a transcriptional coactivator, WWTR1 interacts with transcription factors such as TEADs to regulate genes involved in cell proliferation, differentiation, and apoptosis. Its activity is tightly regulated by phosphorylation-dependent nucleocytoplasmic shuttling, mediated by upstream kinases (e.g., LATS1/2) in the Hippo pathway. Dysregulation of WWTR1 is linked to various cancers, fibrosis, and stem cell maintenance, making it a therapeutic target of interest.
Recombinant WWTR1 protein is engineered for in vitro studies to dissect its molecular interactions, structural features, and signaling mechanisms. Typically produced in bacterial or mammalian expression systems, the recombinant protein retains functional domains, including the WW domain (for protein-protein interactions) and the transcriptional activation domain. It often includes affinity tags (e.g., His-tag) for purification and detection. Researchers use recombinant WWTR1 to investigate its binding partners (e.g., YAP, TEADs), post-translational modifications (e.g., phosphorylation), and responses to pathway modulators. Its applications extend to drug discovery screens, structural biology (e.g., crystallography), and mechanistic studies in cancer biology and regenerative medicine. Quality validation (e.g., mass spectrometry, functional assays) ensures its biological activity for reproducible experimental outcomes.
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