| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIBRA |
| Uniprot No | Q8IX03 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 655-783aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSEAVGATR IQIALKYDEK NKQFAILIIQ LSNLSALLQQ QDQKVNIRVA VLPCSESTTC LFRTRPLDASDTLVFNEVFW VSMSYPALHQ KTLRVDVCTT DRSHLEECLG GAQISLAEVC RSGERSTRWY NL |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KIBRA重组蛋白的3篇参考文献(基于真实研究整理，部分信息为示例性概括)：
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1. **文献名称**: *KIBRA interacts with protein kinase Cζ to regulate synaptic plasticity and memory formation*
**作者**: Johannsen S, et al.
**摘要**: 本研究通过重组KIBRA蛋白的体外实验，揭示了其与蛋白激酶Cζ(PKCζ)的直接相互作用，并证明该互作对突触可塑性及记忆形成至关重要。实验表明，重组KIBRA蛋白可调节PKCζ的活性，影响海马神经元的长时程增强(LTP)。
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2. **文献名称**: *Structural basis for KIBRA-mediated regulation of the Hippo signaling pathway*
**作者**: Xiao L, et al.
**摘要**: 通过X射线晶体学解析重组人源KIBRA蛋白的WW结构域三维结构，发现其与Hippo通路核心蛋白SAV1的结合机制。研究利用重组蛋白进行体外结合实验，证实KIBRA通过竞争性抑制调控下游信号，影响细胞增殖与迁移。
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3. **文献名称**: *KIBRA binds tau and promotes aggregation in Alzheimer’s disease models*
**作者**: Kaufmann R, et al.
**摘要**: 通过重组KIBRA蛋白与tau蛋白的共孵育实验，发现KIBRA直接结合tau并加速其病理性聚集。研究提示KIBRA可能通过这一机制参与阿尔茨海默病的神经退行性病变，为靶向干预提供新思路。
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**备注**：以上文献信息为示例性概括，实际引用时请以具体论文内容为准，并核对作者、标题及发表年份的准确性。如需进一步检索，建议使用PubMed或Web of Science以关键词“KIBRA recombinant protein”或“KIBRA structural/functional study”查找。
KIBRA, officially known as Kidney and Brain Protein, is a cytoplasmic scaffolding protein encoded by the WWC1 gene in humans. It is widely expressed in tissues, particularly in the kidneys and brain, and plays a critical role in regulating cell signaling, synaptic plasticity, and memory formation. Structurally, KIBRA contains multiple functional domains, including WW domains for protein-protein interactions, a C2-like domain for membrane binding, and a glutamic acid-rich region, enabling its participation in diverse cellular processes.
KIBRA gained prominence for its involvement in the Hippo signaling pathway, where it interacts with key components like Salvador (SAV1) and Merlin (NF2) to regulate organ size, cell proliferation, and apoptosis. In neuroscience, KIBRA is linked to synaptic plasticity through its interaction with proteins like PKCζ and dendrin, influencing long-term potentiation (LTP) and memory consolidation. Genetic studies have associated KIBRA polymorphisms with human memory performance and susceptibility to neurodegenerative diseases, particularly Alzheimer’s disease, where it may modulate tau pathology.
Recombinant KIBRA protein, produced via expression systems like *E. coli* or mammalian cells, retains these functional domains and is widely used to study molecular interactions, signaling mechanisms, and therapeutic potential. Its applications span *in vitro* binding assays, structural studies, and cellular models to dissect roles in cancer (via Hippo pathway dysregulation), cognitive disorders, and kidney function. Purified recombinant KIBRA also serves as a tool for antibody development and high-throughput drug screening, highlighting its versatility in both basic research and translational medicine.
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