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产品描述：人血管紧张素Ⅱ受体2抗体(AT2R-Ab)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的AT2R Ab含量。试剂盒检测范围为0.312 U/mL-20 U/mL，适用于科研领域探索AT2R在生理病理过程中的作用机制；其抗体水平变化可能与心血管疾病、纤维化疾病等研究相关；适用于基础研究中评估疾病模型动物的抗体动态变化、体外药物干预实验的效应分析，或筛选调控AT2R信号通路的潜在化合物；为心血管、肾脏等系统相关分子机制研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：angiotensin 2 receptor 2 antibody, AT2R-Ab
缩写：AT2R Ab
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：0.312 U/mL-20 U/mL
灵敏度：0.078 U/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AT2R-Ab in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 82-105 | ||||||
| 1:2 | Average % | 94 | |||||
| Range % | 84-100 | ||||||
| 1:4 | Average % | 98 | |||||
| Range % | 92-101 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 82-105 | ||||||
回收率：
| The recovery of human AT2R-Ab spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 83-105 | |||||
| EDTA plasma (n=4) | 95 | 85-103 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| U/mlOD1OD2AverageCorrected 202.6352.6592.6472.501 101.9631.9271.9451.799 51.2961.2871.2921.146 2.50.7710.7480.7600.614 1.250.4680.4920.4800.334 0.6250.3460.3510.3490.203 0.3120.2550.2460.2510.105 00.1470.1450.146 | |||||||
货期：3-5 working days
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