Human acetylcholine receptor antibody,AChRab ELISA Kit

货号: IPD-E08371h

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人乙酰胆碱受体抗体(AChRab)酶联免疫试剂盒为间接法ELISA试剂盒，定性检测血清样本中的AChR Ab含量。AChR Ab即乙酰胆碱受体抗体。它与重症肌无力等自身免疫性疾病密切相关，是重要研究靶点。其研究机制主要聚焦于它与乙酰胆碱受体结合后，干扰神经肌肉接头处信号传导，导致肌肉无力等症状，为相关疾病诊断与治疗提供依据。适用于重症肌无力等疾病的发病机制研究、药物干预效果评估或实验动物模型抗体水平检测等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：
AChR Ab
种属：
Homo sapiens (Human)
样本类型：
serum
检测范围：
Request Information
灵敏度：
Request Information
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Neuroscience
测定原理：
qualitative
测定方法：
Indirect

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

A 96-well Assay plate --The 96-well plate has been pre-coated with AChR.
Negative Control (1 x 1ml) --Eliminate false positive
Positive Control (1 x 1ml) --Used to evaluate the validity, stability, and comparability of experimental results.
Sample Diluent (1x 20 ml) --Dilute the sample to an appropriate concentration.
HRP-conjugated anti-human IgG (1 x 10ml)--Bind to the AChRab, and HRP catalyzes the TMB to elicit a chromogenic reaction.
Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate