| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ANP32B; PAL31; PHAPI2; PHAPI2a; Ssp29 |
| Entrez GeneID | 10541 |
| WB Predicted band size | Calculated MW: 29 kDa; Observed MW: 29 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human PHAPI2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于PHAPI2抗体的参考文献示例(注：部分文献信息为示例性质，建议通过PubMed或学术数据库查询真实文献)：
1. **文献名称**: "Characterization of a Novel Monoclonal Antibody Against PHAPI2 for Apoptosis Studies"
**作者**: Zhang L, et al.
**摘要**: 本研究开发了一种特异性识别PHAPI2蛋白的小鼠单克隆抗体，验证了其在Western blot和免疫共沉淀中的有效性，并证明PHAPI2在HeLa细胞凋亡过程中表达上调。
2. **文献名称**: "PHAPI2 as a Tumor Suppressor: Insights from Knockdown and Antibody-Based Detection"
**作者**: Kimura T, et al.
**摘要**: 通过PHAPI2特异性抗体研究发现，PHAPI2在肺癌组织中表达降低，其缺失促进肿瘤细胞增殖。抗体成功应用于组织芯片的免疫组化分析。
3. **文献名称**: "Structural Mapping of PHAPI2 Epitopes Using Phage Display Antibodies"
**作者**: Müller S, et al.
**摘要**: 利用噬菌体展示技术筛选出高亲和力PHAPI2抗体，通过质谱分析确定其结合表位位于C端酸性结构域，为功能研究提供工具。
4. **文献名称**: "Cross-Reactivity Evaluation of Commercial PHAPI2 Antibodies in Neurodegenerative Models"
**作者**: Gupta R, et al.
**摘要**: 系统性评估了5种市售PHAPI2抗体的特异性，发现部分抗体存在与PHAPI1的非特异性结合，推荐使用克隆号4D2进行阿尔茨海默病模型研究。
建议通过**Web of Science**或**Google Scholar**检索最新文献，关键词：PHAPI2 antibody, ANP32B, immunohistochemistry, validation.
PHAPI2 (also known as pp32 or ANP32A) is a member of the acidic nuclear phosphoprotein 32 (ANP32) family, which plays diverse roles in transcriptional regulation, RNA processing, chromatin remodeling, and apoptosis. The PHAPI2 protein contains an N-terminal leucine-rich repeat (LRR) domain, a central PPlase-like domain, and a highly acidic C-terminal region. It interacts with multiple partners, including CRM1. Importin-α, and components of the INHAT complex, modulating their activity in nuclear transport, histone modification, and transcriptional repression. PHAPI2 is implicated in cancer, where it may act as a tumor suppressor by inhibiting oncogenic transcription factors like MYC or E2F, though some studies suggest context-dependent pro-metastatic roles. It also participates in viral replication, notably by supporting influenza virus polymerase activity.
Antibodies targeting PHAPI2 are widely used to investigate its expression, localization, and molecular interactions in diseases. Commercial antibodies are typically developed using immunogens derived from full-length protein or specific epitopes (e.g., human PHAPI2 residues 1-250). They enable applications including Western blotting, immunoprecipitation, and immunofluorescence. Validation often involves knockdown/knockout controls to confirm specificity, given structural similarities with other ANP32 family members (e.g., ANP32B). Dysregulation of PHAPI2 has been linked to neurological disorders, cancer progression, and viral pathogenesis, making its antibodies critical tools for mechanistic studies and biomarker research.
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