| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PFKP; PFKF; 6-phosphofructokinase type C; 6-phosphofructokinase; platelet type; Phosphofructo-1-kinase isozyme C; PFK-C; Phosphofructokinase 1; Phosphohexokinase |
| Entrez GeneID | 5214 |
| WB Predicted band size | Calculated MW: 86 kDa; Observed MW: 86 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human PFKP |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于PFKP抗体的3篇示例参考文献(内容为虚构，仅作格式示例)：
1. **文献名称**：*PFKP Expression in Glioblastoma: Role in Glycolytic Adaptation and Prognostic Significance*
**作者**：Smith A, et al.
**摘要**：研究利用PFKP抗体通过免疫组化分析胶质母细胞瘤样本，发现PFKP高表达与肿瘤糖酵解活性增强及患者预后不良相关，提示其作为治疗靶点的潜力。
2. **文献名称**：*Interaction of PFKP with HIF-1α in Hypoxic Tumor Microenvironments*
**作者**：Chen L, et al.
**摘要**：通过免疫共沉淀(使用PFKP抗体)和分子实验，揭示PFKP在缺氧条件下与HIF-1α结合，促进癌细胞糖酵解重编程，驱动肿瘤生长。
3. **文献名称**：*Development of a Novel Monoclonal PFKP Antibody for Metabolic Studies*
**作者**：Kim J, et al.
**摘要**：报道一种高特异性PFKP单克隆抗体的开发，验证其在Western blot、免疫荧光中的可靠性，并应用于肺癌细胞代谢机制研究。
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如需真实文献，建议通过PubMed或Google Scholar搜索“PFKP antibody”或“PFKP in cancer”，筛选涉及该抗体应用的实验研究(如检测表达、功能机制等)。
**Background of PFKP Antibody**
Phosphofructokinase, platelet type (PFKP), is a critical enzyme in glycolysis, catalyzing the phosphorylation of fructose-6-phosphate to fructose-1.6-bisphosphate, a rate-limiting step in this metabolic pathway. As one of three PFK isoforms (PFKP, PFKM, PFKL), PFKP is predominantly expressed in platelets, brain, and placenta, and forms tissue-specific heterotetramers to regulate glycolytic flux. Its activity is tightly controlled by allosteric effectors (e.g., ATP, citrate) and hormonal signals, linking energy metabolism to cellular demands.
PFKP dysregulation is implicated in cancer, metabolic disorders, and neurodegenerative diseases. In tumors, PFKP overexpression often supports the Warburg effect, enhancing aerobic glycolysis to fuel rapid proliferation. It also interacts with non-metabolic pathways, influencing cell survival and signaling.
PFKP antibodies are essential tools for studying its expression, localization, and function. They enable detection of PFKP in western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), aiding research into metabolic reprogramming in diseases. Specific monoclonal or polyclonal antibodies help assess PFKP’s role in therapeutic contexts, such as targeting cancer metabolism. Validation via knockout models or siRNA is crucial to ensure antibody specificity, given high homology among PFK isoforms. These antibodies are pivotal in advancing understanding of PFKP’s dual metabolic and non-canonical roles in health and disease.
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